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AB109516

Anti-Villin antibody [EPR3490]

5

(1 리뷰)

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(7 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Villin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

대체 명칭 보기

VIL, VIL1, Villin-1

2 이미지
Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)

Intracellular flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-Villin antibody [EPR3490] (AB109516)
  • WB

Unknown

Western blot - Anti-Villin antibody [EPR3490] (AB109516)

All lanes:

Western blot - Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

Lane 1:

Human small intestine lysate at 10 µg

Lane 2:

HT-29 lysate at 10 µg

Lane 3:

Caco-2 lysate at 10 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Villin antibody [EPR3490] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3490

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB218331

Anti-Villin antibody [VIL1/1314]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [VIL1/1314] (AB218331)

  • 0
  • 1
Primary Antibodies

AB268129

Anti-Villin antibody [rVIL1/1325]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] (AB268129)

  • 0
  • 0
Primary Antibodies

AB97512

Anti-Villin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB97512)

  • 4
  • 1
Primary Antibodies

AB244291

Anti-Villin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244291)

  • 0
  • 0
Primary Antibodies

AB201989

Anti-Villin antibody [3E5G11] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (AB201989)

  • 4
  • 5
Primary Antibodies

AB130751

Anti-Villin antibody [SP145]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] (AB130751)

  • 5
  • 5
Primary Antibodies

AB233155

Anti-Villin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB233155)

  • 0
  • 0
Primary Antibodies

AB133510

Anti-Villin antibody [EPR3491(3)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [EPR3491(3)] (AB133510)

  • 0
  • 0
Primary Antibodies

AB202021

Anti-Villin antibody [5E3B2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (AB202021)

  • 0
  • 0
Primary Antibodies

AB244292

Anti-Villin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (AB244292)

  • 0
  • 0
Primary Antibodies

AB245749

Anti-Villin antibody [SP145] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] - BSA and Azide free (AB245749)

  • 0
  • 0

보완 제품

Primary Antibodies

AB108508

Anti-Lysozyme antibody [EPR2994(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (AB108508)

  • 5
  • 4
Primary Antibodies

AB76126

Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker (AB76126)

  • 5
  • 17
Primary Antibodies

AB11197

Anti-MUC2 antibody [996/1]

BOND RX™ Validated|Recombinant

Immunohistochemistry - Anti-MUC2 antibody [996/1] (AB11197)

  • 4
  • 5
Primary Antibodies

AB283265

Anti-Chromogranin A antibody [RM1025]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [RM1025] (AB283265)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

EBioMedicine 104:105181 PubMed38838469

2024

Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeru Fujita,Arnon Plianchaisuk,Sayaka Deguchi,Hayato Ito,Naganori Nao,Lei Wang,Hesham Nasser,Tomokazu Tamura,Izumi Kimura,Yukie Kashima,Rigel Suzuki,Saori Suzuki,Izumi Kida,Masumi Tsuda,Yoshitaka Oda,Rina Hashimoto,Yukio Watanabe,Keiya Uriu,Daichi Yamasoba,Ziyi Guo,Alfredo A Hinay,Yusuke Kosugi,Luo Chen,Lin Pan,Yu Kaku,Hin Chu,Flora Donati,Sarah Temmam,Marc Eloit,Yuki Yamamoto,Tetsuharu Nagamoto,Hiroyuki Asakura,Mami Nagashima,Kenji Sadamasu,Kazuhisa Yoshimura,Yutaka Suzuki,Jumpei Ito,Terumasa Ikeda,Shinya Tanaka,Keita Matsuno,Takasuke Fukuhara,Kazuo Takayama,Kei Sato

Biotechnology journal 19:e2300390 PubMed38375564

2024

A generic pump-free organ-on-a-chip platform for assessment of intestinal drug absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiong Guo,Yingying Xie,Jianhua Qin

Tissue engineering and regenerative medicine 19:403-415 PubMed35122584

2022

Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui Yang

Molecular therapy. Methods & clinical development 17:49-57 PubMed31890740

2020

Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Kanae Kawai,Ryosuke Negoro,Moe Ichikawa,Tomoki Yamashita,Sayaka Deguchi,Kazuo Harada,Kazumasa Hirata,Kazuo Takayama,Hiroyuki Mizuguchi

Scientific reports 8:16393 PubMed30401824

2018

Aggregated SOD1 causes selective death of cultured human motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Benkler,Alison L O'Neil,Susannah Slepian,Fang Qian,Paul H Weinreb,Lee L Rubin

Biochemical and biophysical research communications 472:631-6 PubMed26966071

2016

Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells.

Applications

Flow Cyt

Species

Human

Ryosuke Negoro,Kazuo Takayama,Yasuhito Nagamoto,Fuminori Sakurai,Masashi Tachibana,Hiroyuki Mizuguchi

Scientific reports 5:16479 PubMed26559489

2015

Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine.

Applications

Flow Cyt

Species

Human

Tatsuya Ozawa,Kazuo Takayama,Ryota Okamoto,Ryosuke Negoro,Fuminori Sakurai,Masashi Tachibana,Kenji Kawabata,Hiroyuki Mizuguchi
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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