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AB188499

Anti-Vimentin antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.
3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.

Western blot - Anti-Vimentin antibody (AB188499)
  • WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB188499)

All lanes:

Western blot - Anti-Vimentin antibody (ab188499) at 1/5000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 4:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Predicted band size: 53 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VIM.

P08670

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대체품

Primary Antibodies

AB203428

Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB203428)

  • 0
  • 0
Primary Antibodies

AB154207

Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

  • 0
  • 0
Primary Antibodies

AB185030

Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB185030)

  • 5
  • 2
Primary Antibodies

AB194719

Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194719)

  • 5
  • 1
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB52942

Anti-Vimentin (phospho S39) antibody [EP1069Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)

  • 3
  • 1
Primary Antibodies

AB193555

Anti-Vimentin antibody [EPR3776] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - BSA and Azide free (AB193555)

  • 5
  • 4

보완 제품

Assay Kits

AB246526

Human Vimentin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

  • 0
  • 0
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purity
Whole antiserum
보관 버퍼
Preservative: 0.07% Sodium azide
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
See full target information VIM

대체 명칭 보기

Vimentin, VIM
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal of virology 97:e0087823 PubMed37905840

2023

Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Pahmeier,Teresa-Maria Lavacca,Sarah Goellner,Christopher J Neufeldt,Vibhu Prasad,Berati Cerikan,Sreejith Rajasekharan,Giulia Mizzon,Uta Haselmann,Charlotta Funaya,Pietro Scaturro,Mirko Cortese,Ralf Bartenschlager

Cell host & microbe 28:853-866.e5 PubMed33245857

2020

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Cortese,Ji-Young Lee,Berati Cerikan,Christopher J Neufeldt,Viola M J Oorschot,Sebastian Köhrer,Julian Hennies,Nicole L Schieber,Paolo Ronchi,Giulia Mizzon,Inés Romero-Brey,Rachel Santarella-Mellwig,Martin Schorb,Mandy Boermel,Karel Mocaer,Marianne S Beckwith,Rachel M Templin,Viktoriia Gross,Constantin Pape,Christian Tischer,Jamie Frankish,Natalie K Horvat,Vibor Laketa,Megan Stanifer,Steeve Boulant,Alessia Ruggieri,Laurent Chatel-Chaix,Yannick Schwab,Ralf Bartenschlager

The Journal of pharmacology and experimental thera 370:416-426 PubMed31248980

2019

Platelet-Specific Deletion of Cyclooxygenase-1 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Sacco,Annalisa Bruno,Annalisa Contursi,Melania Dovizio,Stefania Tacconelli,Emanuela Ricciotti,Paloma Guillem-Llobat,Tania Salvatore,Luigia Di Francesco,Rosa Fullone,Patrizia Ballerini,Vincenzo Arena,Sara Alberti,Guizhu Liu,Yanjun Gong,Alessandro Sgambato,Carlo Patrono,Garret A FitzGerald,Ying Yu,Paola Patrignani

Molecular medicine reports 18:2753-2761 PubMed30015869

2018

miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/β-catenin pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Liwei Sun,Jinduo Li,Rong Jiang

Oncology research 26:529-536 PubMed28653599

2017

[ARTICLE WITHDRAWN] MicroRNA-539 Inhibits the Epithelial-Mesenchymal Transition of Esophageal Cancer Cells by Twist-Related Protein 1-Mediated Modulation of Melanoma-Associated Antigen A4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhili Cao,Xiang Zheng,Lei Cao,Naixin Liang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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