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AB242271

violetFluor™ 450 Anti-CD19 antibody [HIB19]

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Anti-CD19 antibody [HIB19] conjugated to violetFluor™ 450 - suitable for flow cytometry, and reacts with human samples. Clone [HIB19] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - violetFluor™ 450 Anti-CD19 antibody [HIB19] (AB242271)
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Supplier Data

Flow Cytometry - violetFluor™ 450 Anti-CD19 antibody [HIB19] (AB242271)

Flow cytometric analysis of human peripheral blood lymphocytes staining CD19 using 5 μl (0.5 μg) ab242271 (solid line) or 0.5 μg violetFluor™ 450 Mouse IgG1 isotype control.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HIB19

Isotype

IgG1

Light chain 타입

kappa

Conjugation

violetFluor™ 450

Excitation/Emission

Ex: 405nm, Em: 450nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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대체품

Primary Antibodies

AB134114

Anti-CD19 antibody [EPR5906]

KO Validated|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD19 antibody [EPR5906] (AB134114)

  • 4
  • 5
Primary Antibodies

AB195896

HRP Anti-CD19 antibody [EPR5906]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD19 antibody [EPR5906] (AB195896)

  • 0
  • 0
Primary Antibodies

AB196468

Alexa Fluor® 488 Anti-CD19 antibody [EPR5906]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD19 antibody [EPR5906] (AB196468)

  • 2
  • 3
Primary Antibodies

AB196515

Alexa Fluor® 647 Anti-CD19 antibody [EPR5906]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD19 antibody [EPR5906] (AB196515)

  • 5
  • 1
Primary Antibodies

AB215382

Anti-CD19 antibody [EPR5906] - Low endotoxin, Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD19 antibody [EPR5906] - Low endotoxin, Azide free (AB215382)

  • 0
  • 0
Primary Antibodies

AB225717

Anti-CD19 antibody [SP110] - C-terminal

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP110] - C-terminal (AB225717)

  • 0
  • 0
Primary Antibodies

AB237772

Anti-CD19 antibody [SP291] - BSA and Azide free

KO Validated|BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - BSA and Azide free (AB237772)

  • 0
  • 0
Primary Antibodies

AB274888

Alexa Fluor® 555 Anti-CD19 antibody [EPR5906]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD19 antibody [EPR5906] (AB274888)

  • 5
  • 1

보완 제품

Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB302698

Alexa Fluor® 647 Anti-CD14 antibody [SP192]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 5 μl (0.5 μg)/test.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
보관 버퍼
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.
Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).
See full target information CD19

대체 명칭 보기

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 27:109439 PubMed38523774

2024

A tetravalent nanovaccine that inhibits growth of HPV-associated head and neck carcinoma via dendritic and T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Romano Josi,Daniel E Speiser,Simone de Brot,Anne-Cathrine Vogt,Eva M Sevick-Muraca,Genrich V Tolstonog,Martin F Bachmann,Mona O Mohsen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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