AB241097
violetFluor™ 450 Anti-CD4 antibody [RM4-5]
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(2 제품이 사용된 논문 )
Anti-CD4 antibody [RM4-5] conjugated to violetFluor™ 450 - suitable for flow cytometry, and reacts with mouse samples. Clone [RM4-5] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.
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1 이미지
- Flow Cyt
Supplier Data
Flow Cytometry - violetFluor™ 450 Anti-CD4 antibody [RM4-5] (AB241097)
Flow cytometric analysis of C57Bl/6 splenocytes stained for CD4 with 0.125 μg ab241097 (solid line) or 0.125 μg violetFluor™ 450 Rat IgG2a (dashed line).
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.125 μg.</p>" } } }
제품 세부 정보
If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will compete for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
특성 및 보관 정보
제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
보관 버퍼
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Store in the dark
제품 프로토콜
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
대체 명칭 보기
CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
제품이 사용된 논문 (2)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Neoplasma 69:1041-1053 PubMed35723199
2022
Exosomes from M2 macrophages promoted glycolysis in FaDu cells by inhibiting PDLIM2 expression to stabilize PFKL.
Applications
Unspecified application
Species
Unspecified reactive species
Peng Wang,Guang-Yao Li,Lei Zhou,Huai-Li Jiang,Yue Yang,Hai-Tao Wu
NPJ vaccines 7:24 PubMed35197485
2022
An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission.
Applications
Unspecified application
Species
Unspecified reactive species
Kairat Tabynov,Nurkeldi Turebekov,Meruert Babayeva,Gleb Fomin,Toktassyn Yerubayev,Tlektes Yespolov,Lei Li,Gourapura J Renukaradhya,Nikolai Petrovsky,Kaissar Tabynov
Product promise
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