Anti-Viperin antibody [MaP.VIP] - BSA and Azide free

• IP

Lab

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Viperin was immunoprecipitated from 0.35 mg KG-1a (human bone marrow myeloblast) whole cell lysate with ab107359 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

Lane 1 : KG-1a (human bone marrow myeloblast) whole cell lysate
Lane 2 : ab107359 IP in KG-1a (human bone marrow myeloblast) whole cell lysate
Lane 3 : Mouse IgG2a, kappaIsotype control (ab18413) instead of ab107359 in KG-1a whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 93 seconds.

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/ko/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

KG-1a (human bone marrow myeloblast) whole cell lysate (Input) at 10 µg

Lane 2:

KG-1a (human bone marrow myeloblast) whole cell lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/ko/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/ko/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/ko/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Viperin with ab107359 at 1/500 (1.966 μg/ml) dilution , followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in Raw264.7 cells (shown in green) treated with 100 ng/ml LPS (lipopolysaccharides) and 100 U/ml IFN gamma for 4 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• IP

Lab

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Viperin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab107359 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

Lane 1 : Mouse spleen tissue lysate
Lane 2 : ab107359 IP in Mouse spleen tissue lysate
Lane 3 : Mouse IgG2a, kappaIsotype control (ab18413) instead of ab107359 in mouse spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 93 seconds.

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/ko/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

Mouse spleen tissue lysate (Input) at 10 µg

Lane 2:

Mouse spleen tissue lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/ko/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/ko/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/ko/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• WB

Lab

Western blot - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2

Negative control : SW480

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Viperin antibody [MaP.VIP] (<a href='/ko/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/1000 dilution

Lane 1:

HCC1937 (human mammary gland epithelial cell) whole cell lysate at 20 µg

Lane 2:

Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 7:

RAW264.7 treated with 100ng/ml LPS and 100U/ml IFN gamma for 4h whole cell lysate at 20 µg

Lane 8:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 9:

THP-1 treated with 100ng/ml LPS and 100U/ml IFN gamma for 4h whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Viperin antibody [MaP.VIP] (<a href='/ko/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 42 kDa

false

Exposure time: 10s