AB3508

Anti-Vitamin D Receptor antibody

Name: Anti-Vitamin D Receptor antibody (ab3508) | Abcam
Brand: Abcam Limited
SKU: AB3508
Rating: 4 (6 reviews)

(6 리뷰들)

(86 제품이 사용된 논문 )

Rabbit Polyclonal Vitamin D Receptor antibody. Suitable for IP, EMSA, ChIP, WB, IHC-P, ICC/IF, ChIP-seq, IHC-Fr, Gel Shift Assay and reacts with Mouse, Rat, Chicken, Human samples. Cited in 86 publications. Immunogen corresponding to Synthetic Peptide within Human VDR aa 350-450.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (AB3508)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (AB3508)

ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
Left panel : with primary antibody at 1/2000. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Chicken, Mouse

Applications

EMSA, IP, WB, ChIP-seq, Gel Shift Assay, IHC-Fr, ChIP, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human VDR aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P11473

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "EMSA" : {"fullname" : "Electrophoretic Mobility Shift Assay", "shortname":"EMSA"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "GelShiftAssay" : {"fullname" : "Gel Shift Assay", "shortname":"Gel Shift Assay"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "guaranteed", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/4000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "guaranteed", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "guaranteed", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "guaranteed", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "guaranteed", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "guaranteed", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" }, "Chicken": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "guaranteed", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "guaranteed", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "predicted", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "predicted", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" }, "Saguinus oedipus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "EMSA-species-checked": "predicted", "EMSA-species-dilution-info": "", "EMSA-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "GelShiftAssay-species-checked": "predicted", "GelShiftAssay-species-dilution-info": "", "GelShiftAssay-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purity

Whole antiserum

보관 버퍼

Preservative: 0.05% Sodium azide

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed : 10678179, PubMed : 15728261, PubMed : 16913708, PubMed : 28698609, PubMed : 37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed : 28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed : 28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed : 12016314, PubMed : 32354638).

See full target information VDR

대체 명칭 보기

NR1I1, VDR, Vitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1

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제품이 사용된 논문 (86)

Recent publications for all applications. Explore the 전체 목록and refine your search

International journal of veterinary science and medicine 13:1-9 PubMed40290667

2025

Post-operative supplementation with vitamin D after mucogingival surgery significantly enhances autophagy and improves life quality following feline chronic gingivostomatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Kai Huang,Chung-Wei Chen,Ting-Yi Renn,Ming-Yi Shen,Igor V Klimenkov,Nikolay P Sudakov,Sandeep Kumar Singh,Hung-Ming Chang

Frontiers in cell and developmental biology 13:1545167 PubMed40276652

2025

Loss of vitamin D receptor induces premature ovarian insufficiency through compromising the 7-dehydrocholesterol-dependent anti-aging effects.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Chen,Qiuyi Wang,Yi Zhang,Luxi Shangguan,Zhengquan Zhu,Huan Zhang,Xiang Zou,Qinghe Geng,Yanting Wen,Daojuan Wang,Yong Wang

International journal of molecular sciences 26: PubMed40243431

2025

Placental Molecular Expression of Different Pathogenic Vaginal Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Roxana Matasariu,Constantin Condac,Victoria Bîrluțiu,Ludmila Lozneanu,Iuliana Elena Bujor,Vasile Lucian Boiculese,Mihai Sava,Alexandra Ursache

Aging 17:1026-1042 PubMed40168539

2025

Mesenchymal stem cell-specific Sirt1 overexpression prevents sarcopenia induced by 1,25-dihydroxyvitamin D deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Chen,Biqi Ren,Jing Wang,Xingchen Liu,Xiangjiao Yi,David Goltzman,Dengshun Miao

Redox biology 80:103518 PubMed39891958

2025

Protective role of vitamin D receptor against mitochondrial calcium overload from PM-Induced injury in renal tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqiu Lu,Zishun Zhan,Dan Li,Hengbing Chen,Aimei Li,Jing Hu,Zhijun Huang,Bin Yi

Frontiers in immunology 15:1466802 PubMed39840066

2025

Restoring natural killer cell activity in lung injury with 1,25-hydroxy vitamin D: a promising therapeutic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Mohammad Abuawad

BMC nephrology 26:18 PubMed39799338

2025

Risk factors for radial artery calcification in patients with and without uremia.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Bing Hao,Si-Yu Wang,Tong Chen,Bo Yuan,Li-Rong Hao

Biomolecules 14: PubMed39766282

2025

APOL1 Modulates Renin-Angiotensin System.

Applications

Unspecified application

Species

Unspecified reactive species

Vinod Kumar,Prabhjot Kaur,Kameshwar Ayasolla,Alok Jha,Amen Wiqas,Himanshu Vashistha,Moin A Saleem,Waldemar Popik,Ashwani Malhotra,Christoph A Gebeshuber,Karl Skorecki,Pravin C Singhal

Journal of clinical medicine 13: PubMed39458089

2024

Vitamin D Receptor-Interplay in COVID-19-Negative, -Infected, and -Vaccinated Women during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Constantin Condac,Ludmila Lozneanu,Daniela Roxana Matasariu,Alexandra Ursache,Iuliana Elena Bujor,Maria Elena Niță,Vasile Lucian Boiculese,Mihai Sava,Paula Țăroi,Victoria Bîrluțiu

Frontiers in physiology 14:1322677 PubMed38264331

2024

Active vitamin D increases myogenic differentiation in C2C12 cells via a vitamin D response element on the myogenin promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn H Alliband,Tim Parr,Preeti H Jethwa,John M Brameld

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-Vitamin D Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (AB3508)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (86)

Product promise