Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free
- RabMAb
- Recombinant
- 각 태그의 뜻
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Rabbit Recombinant Monoclonal Vitamin D3 antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.
대체 명칭 보기
cholecalciferol
- cELISA
Supplier Data
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (AB236470)
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
- cELISA
Supplier Data
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (AB236470)
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-Vitamin D3 antibody [EPPTX-R6-42-12]
Reactivity 정보
제품 세부 정보
ab236470 is the carrier-free version of ab229820.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
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보관 정보
제품 프로토콜
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제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Genes & genomics 47:961-972 PubMed40794264
2025
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com