AB46808

Anti-Vitronectin/S-Protein antibody [EP781Y]

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Vitronectin/S-Protein antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

This image shows human lung tissue treated with ab46808 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Purified ab46808 at 1/50 dilution (2μg) immunoprecipitating Vitronectin/S-Protein in Human serum.
Lane 1 (input) : Human serum 10μg
Lane 2 (+) : ab46808 + Human serum.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 65-75 kDa

All lanes:

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

Predicted band size: 54 kDa

false

Unknown

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

All lanes:

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Goat anti-rabbit, HRP labelled. at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa,60 kDa,75 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP781Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

See full target information Vitronectin

대체 명칭 보기

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

style

left-column

제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The American journal of pathology 194:180-194 PubMed38029923

2023

Multilamellated Basement Membranes in the Capillary Network of Alveolar Capillary Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jan C Kamp,Lavinia Neubert,Jonas C Schupp,Peter Braubach,Christoph Wrede,Florian Laenger,Tim Salditt,Jakob Reichmann,Tobias Welte,Arjang Ruhparwar,Fabio Ius,Nicolaus Schwerk,Anke K Bergmann,Sandra von Hardenberg,Matthias Griese,Christina Rapp,Karen M Olsson,Jan Fuge,Da-Hee Park,Marius M Hoeper,Danny D Jonigk,Lars Knudsen,Mark P Kuehnel

Endocrine journal 68:543-552 PubMed33473055

2021

Circular RNA circ_0008274 enhances the malignant progression of papillary thyroid carcinoma via modulating solute carrier family 7 member 11 by sponging miR-154-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Ma,Zhenghua Kan

Ultrasound in medicine & biology 45:3102-3115 PubMed31537389

2019

Mechanical Weakness of Thoracic Aorta Related to Aging or Dissection Predicted by Speed of Sound with Collagenase.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Miura,Kanna Yamashita

International journal of clinical and experimental 8:11364-71 PubMed26617861

2015

Vitronectin significantly influences prognosis in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Shi,Rui-Long Lan,Xuan Tao,Chao-Yang Wu,Hai-Feng Hong,Jian-Hua Lin

Molecular medicine reports 7:53-8 PubMed23124223

2012

Increased expression levels of vitronectin in the maternal‑fetal interface of placenta in early-onset severe preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shen,Xiaoyan Ma,Fei Yi,Azhar Rasul,Manhua Cui,Tonghui Ma

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vitronectin/S-Protein antibody [EP781Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise