AB140989

Anti-Von Hippel Lindau/VHL antibody [OTI1E1]

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(15 제품이 사용된 논문 )

Mouse Monoclonal Von Hippel Lindau/VHL antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VHL.

대체 명칭 보기

von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human endometrium tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human bladder tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human ovary adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human lung carcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human pancreas tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Paraffin-embedded human colon adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at 1/150 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

All lanes:

Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (ab140989) at 1/4000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY Von Hippel Lindau cDNA at 5 µg

Predicted band size: 24 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1E1

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VHL.

P40337

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Clone OTI1E1 (formerly 1E1).

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).

See full target information VHL

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제품이 사용된 논문 (15)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The EMBO journal 43:931-955 PubMed38360997

2024

VHL suppresses autophagy and tumor growth through PHD1-dependent Beclin1 hydroxylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Meisi Yan,Leiguang Ye,Qimin Zhou,Yuran Duan,Hongfei Jiang,Lei Wang,Yuan Ouyang,Huahe Zhang,Yuli Shen,Guimei Ji,Xiaohan Chen,Qi Tian,Liwei Xiao,Qingang Wu,Ying Meng,Guijun Liu,Leina Ma,Bo Lei,Zhimin Lu,Daqian Xu

Cell research 34:13-30 PubMed38163844

2024

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzi Mao,Jiaojiao Zhang,Qian Zhou,Xiadi He,Zhifang Zheng,Yun Wei,Kaiqiang Zhou,Yan Lin,Haowen Yu,Haihui Zhang,Yineng Zhou,Pengcheng Lin,Baixing Wu,Yiyuan Yuan,Jianyuan Zhao,Wei Xu,Shimin Zhao

Journal of immunology (Baltimore, Md. : 1950) 211:261-273 PubMed37314413

2023

Nicotinamide Antagonizes Lipopolysaccharide-Induced Hypoxic Cell Signals in Human Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Colleen S Curran,Edward J Dougherty,Xizhong Cui,Yan Li,Mark Jeakle,Tom Gamble,Cumhur Y Demirkale,Parizad Torabi-Parizi

Journal of inflammation research 15:6553-6567 PubMed36510494

2022

The Infiltration of Neutrophil Granulocytes Due to Loss of PTEN Was Associated with Poor Response to Immunotherapy in Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Wu,Jie Chen,Kang Yao,Daming Fan,Minglei Wang,Yongjun Liu,Shouhu Xin,Zeqiang Sun,Shun Li,Yang Sun,Qingyong Liu

Frontiers in oncology 11:654812 PubMed34136390

2021

Depletion Induces HIF Activity in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sumei Yao,Ayse Ertay,Yilu Zhou,Liudi Yao,Charlotte Hill,Jinliang Chen,Yangbo Guan,Hui Sun,Rob M Ewing,Yifei Liu,Xuedong Lv,Yihua Wang

Pathology, research and practice 224:153490 PubMed34098199

2021

Clinicopathological features of sclerosing angiomatoid nodular transformation of the spleen.

Applications

Unspecified application

Species

Unspecified reactive species

Hongqun Wang,Bingrong Hu,Wei Chen,Jiaming Ren,Peng Liu,Huaiyin Shi

Cancers 13: PubMed33801954

2021

Neo-Fs Index: A Novel Immunohistochemical Biomarker Panel Predicts Survival and Response to Anti-Angiogenetic Agents in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jisup Kim,Jee-Young Park,Su-Jin Shin,Beom Jin Lim,Heounjeong Go

Gene 754:144851 PubMed32525044

2020

The histone deacetylase HDAC1 activates HIF1α/VEGFA signal pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Meng Wei,Chao Wang,Danping Sun,Peng Liu,Xin Zhong,Qingsi He,Wenbin Yu

Molecular medicine reports 21:1011-1020 PubMed31922242

2020

miR‑155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xi,Qiang-Qiang Li,Bing-Qiang Li,Ning Li

Precision clinical medicine 2:140-151 PubMed31598385

2019

Establishment of xenografts of urological cancers on chicken chorioallantoic membrane (CAM) to study metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Junhui Hu,Moe Ishihara,Arnold I Chin,Lily Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Von Hippel Lindau/VHL antibody [OTI1E1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (AB140989)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (15)

Product promise