AB183312

Anti-VPAC1 antibody [SP234]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal VPAC1 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 2 publications.

대체 명칭 보기

Vasoactive intestinal polypeptide receptor 1, VIP-R-1, Pituitary adenylate cyclase-activating polypeptide type II receptor, VPAC1 receptor, PACAP type II receptor, PACAP-R-2, PACAP-R2, VPAC1R, VIPR1

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for VPAC1 using ab183312 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-VPAC1 antibody [SP234] (AB183312)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPAC1 antibody [SP234] (AB183312)

Flow cytometric analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VPAC1 using ab183312 at 1/100 dilution (green) compared to a negative control rabbit IgG (blue)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP234

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Primary antibody incubation for 10 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "Primary antibody incubation for 30 minutes at 4°C." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Primary antibody incubation for 10 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS by protein A/G.

보관 버퍼

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G protein-coupled receptor activated by the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (ADCYAP1/PACAP) (PubMed : 35477937, PubMed : 36385145, PubMed : 8179610). Binds VIP and both PACAP27 and PACAP38 bioactive peptides with the following order of ligand affinity VIP = PACAP27 > PACAP38 (PubMed : 35477937, PubMed : 8179610). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway (PubMed : 35477937, PubMed : 36385145, PubMed : 8179610).

See full target information Vasoactive intestinal polypeptide receptor 1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cells translational medicine 14: PubMed40371959

2025

Immune rejection of human mesenchymal stem cells compared to extracellular vesicles in mice with renal artery stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tarek Ziad Arabi,Yazan Almasry,Ailing Xue,Alfonso Eirin,Amir Lerman,Xiang-Yang Zhu,Lilach O Lerman

Cells 13: PubMed38391908

2024

Vasoactive Intestinal Peptide Receptor, CRTH2, Antagonist Treatment Improves Eosinophil and Mast Cell-Mediated Esophageal Remodeling and Motility Dysfunction in Eosinophilic Esophagitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chandra Sekhar Yadavalli,Sathisha Upparahalli Venkateshaiah,Alok K Verma,Chandrasekhar Kathera,Pearce S Duncan,Michael Vaezi,Richard J Paul,Anil Mishra

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VPAC1 antibody [SP234]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

Flow Cytometry (Intracellular) - Anti-VPAC1 antibody [SP234] (AB183312)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] (AB183312)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise