Anti-VPAC2 antibody [SP235] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] - BSA and Azide free (AB246352)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling VPAC2 using ab183334 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183334).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] - BSA and Azide free (AB246352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183334)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VPAC2 antibody [SP235] - BSA and Azide free (AB246352)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling VPAC2 with purified ab183334 at 1/20 dilution (5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183334).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPAC2 antibody [SP235] - BSA and Azide free (AB246352)

Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling VPAC2 using ab183334 (green) compare to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183334).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] - BSA and Azide free (AB246352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183334)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] - BSA and Azide free (AB246352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183334)