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AB240574

Anti-VPS18 antibody

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Rabbit Polyclonal VPS18 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human VPS18 aa 1-50.

대체 명칭 보기

KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18

2 이미지
Immunoprecipitation - Anti-VPS18 antibody (AB240574)
  • IP

Supplier Data

Immunoprecipitation - Anti-VPS18 antibody (AB240574)

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lane 1 : ab240574 used for IP at 6 μg per reaction. For WB 1 μg/ml.
Lane 2 : Control IgG.
Chemiluminescence detection : 3 minutes.

All lanes:

Immunoprecipitation - Anti-VPS18 antibody (ab240574)

Predicted band size: 110 kDa

false

Western blot - Anti-VPS18 antibody (AB240574)
  • WB

Supplier Data

Western blot - Anti-VPS18 antibody (AB240574)

All lanes:

Western blot - Anti-VPS18 antibody (ab240574) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 110 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human VPS18 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9P253

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대체품

Primary Antibodies

AB178689

Anti-VPS18 antibody [EPR13379]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13379] (AB178689)

  • 0
  • 0
Primary Antibodies

AB178416

Anti-VPS18 antibody [EPR13378] - N-terminal

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (AB178416)

  • 4
  • 1

보완 제품

Primary Antibodies

AB181078

Anti-VPS41 antibody [EPR13268]

RabMAb|Recombinant

Western blot - Anti-VPS41 antibody [EPR13268] (AB181078)

  • 5
  • 1
Primary Antibodies

AB50533

Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker

Lab Essentials

Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (AB50533)

  • 4
  • 27
Primary Antibodies

AB109110

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)

  • 5
  • 4
Primary Antibodies

AB180835

Anti-CRB3 antibody [14F9]

Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (AB180835)

  • 2
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed : 11382755, PubMed : 23351085, PubMed : 24554770, PubMed : 25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed : 25783203). Involved in dendrite development of Pukinje cells (By similarity).
See full target information VPS18
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 31: PubMed39450567

2024

S100A16 stabilizes the ITGA3‑mediated ECM‑receptor interaction pathway to drive the malignant properties of lung adenocarcinoma cells via binding MOV10.

Applications

Unspecified application

Species

Unspecified reactive species

Lianren Yang,Ajuan Shen,Rujun Wang,Zhihui Zheng
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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