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AB249413

Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal VPS28 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

대체 명칭 보기

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)

This data was developed using ab167172, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS28 with ab167172 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)

This data was developed using ab167172, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling VPS28 with ab167172 at 1/100 dilution.

Western blot - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)
  • WB

Unknown

Western blot - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)

This data was developed using ab167172, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9881(B)] (<a href='/ko/products/primary-antibodies/vps28-antibody-epr9881b-ab167172'>ab167172</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9881(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

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Primary Antibodies

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Primary Antibodies

AB235050

Anti-CHMP6/VPS20 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP6/VPS20 antibody (AB235050)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

ab249413 is the carrier-free version of ab167172.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
See full target information VPS28
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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