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AB137027

Anti-VPS4B/MIG1 antibody [EPR9225]

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(3 제품이 사용된 논문 )

Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) is a rabbit monoclonal antibody detecting VPS4B/MIG1 in Western Blot, Flow Cytometry (Intra). Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

SKD1, VPS42, MIG1, VPS4B, Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1

2 이미지
Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

Intracellular flow cytometric analysis of permeabilized 293T cells labelling VPS4B/MIG1 with ab137027 at 1/10 dilution (red). Rabbit IgG negative control is green.

Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)
  • WB

Unknown

Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (AB137027)

All lanes:

Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 49 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (5)

  • Carrier free

    Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free

  • 660 APC

    APC Anti-VPS4B/MIG1 antibody [EPR9225]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-VPS4B/MIG1 antibody [EPR9225]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VPS4B/MIG1 antibody [EPR9225]

  • 578 PE

    PE Anti-VPS4B/MIG1 antibody [EPR9225]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9225

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

제품 세부 정보

What is this antibody validated in?
Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

What is the molecular weight of VPS4B/MIG1?
Anti-VPS4B/MIG1 [EPR9225] (ab137027) specifically detects a band for VPS4B/MIG1 (UniProt: O75351) at a molecular weight of 49kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR9225] also available for your convenience: ab137027, Carrier free - ab248775, APC - ab319287, PE - ab319435, Alexa Fluor® 488 - ab319569, Alexa Fluor® 647 - ab319682

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purity
Tissue culture supernatant
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed : 18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413).. (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
See full target information VPS4B

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proteomics 22:e2200049 PubMed36037246

2022

Proteomic characteristics of plasma and blood cells in natural aging rhesus monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wu,Fuchuan Xiao,Jing Zhang,Yafei Chi,Yanan Zhai,Baian Chen,Jing Lu

Cell reports 33:108493 PubMed33326793

2020

Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.

Applications

Unspecified application

Species

Unspecified reactive species

Jasper E Neggers,Brenton R Paolella,Adhana Asfaw,Michael V Rothberg,Thomas A Skipper,Annan Yang,Radha L Kalekar,John M Krill-Burger,Neekesh V Dharia,Guillaume Kugener,Jérémie Kalfon,Chen Yuan,Nancy Dumont,Alfredo Gonzalez,Mai Abdusamad,Yvonne Y Li,Liam F Spurr,Westley W Wu,Adam D Durbin,Brian M Wolpin,Federica Piccioni,David E Root,Jesse S Boehm,Andrew D Cherniack,Aviad Tsherniak,Andrew L Hong,William C Hahn,Kimberly Stegmaier,Todd R Golub,Francisca Vazquez,Andrew J Aguirre

Nature cell biology 19:787-798 PubMed28604678

2017

Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis.

Applications

WB

Species

Unspecified reactive species

Beata E Mierzwa,Nicolas Chiaruttini,Lorena Redondo-Morata,Joachim Moser von Filseck,Julia König,Jorge Larios,Ina Poser,Thomas Müller-Reichert,Simon Scheuring,Aurélien Roux,Daniel W Gerlich
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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