AB211358

Anti-VRK1 antibody

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Polyclonal VRK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VRK1 aa 1-150.

대체 명칭 보기

Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody (AB211358)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody (AB211358)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-251 MG cells labeling VRK1 with ab211358 at 4 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRK1 antibody (AB211358)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRK1 antibody (AB211358)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling VRK1 with ab211358 at 1/200 dilution.

Supplier Data

Western blot - Anti-VRK1 antibody (AB211358)

All lanes:

Western blot - Anti-VRK1 antibody (ab211358) at 0.4 µg/mL

Lane 1:

Rh30 cell lysate with siRNA control

Lane 2:

Rh30 cell lysate with siRNA Target specific probe

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-VRK1 antibody (AB211358)

All lanes:

Western blot - Anti-VRK1 antibody (ab211358) at 0.4 µg/mL

All lanes:

HL-60 (Human promyelocytic leukemia) cell lysate

Predicted band size: 45 kDa,73 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VRK1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q99986

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed : 14645249, PubMed : 18617507, PubMed : 19103756, PubMed : 33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed : 31527692, PubMed : 37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed : 33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed : 21920476, PubMed : 31090908, PubMed : 31527692). Involved in Golgi disassembly during the cell cycle : following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed : 19103756). Phosphorylates BANF1 : disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed : 16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed : 10951572, PubMed : 31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed : 15105425). Phosphorylates JUN (PubMed : 31527692).

See full target information VRK1

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in oncology 10:601055 PubMed33330099

2020

Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Min Fang,Minjun Zhang,Yiqing Wang,Fangqiang Wei,Jianhui Wu,Xiaozhou Mou,Yigan Zhang,Xiaodong Liang,Jianming Tang

Oncology letters 20:1163-1170 PubMed32724356

2020

Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Ren,Jie Geng,Chao Xiong,Xuebing Li,Yuqing Li,Jin Li,Hongchun Liu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VRK1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody (AB211358)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRK1 antibody (AB211358)

Western blot - Anti-VRK1 antibody (AB211358)

Western blot - Anti-VRK1 antibody (AB211358)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise