AB109486

Anti-WAC antibody

Name: Anti-WAC antibody
Brand: Abcam Limited
SKU: AB109486
Rating: 4 (1 reviews)

(1 리뷰)

(4 제품이 사용된 논문 )

Rabbit Polyclonal WAC antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

대체 명칭 보기

KIAA1844, WAC, WW domain-containing adapter protein with coiled-coil

2 이미지

ICC/IF

Immunocytochemistry/ Immunofluorescence - Anti-WAC antibody (AB109486)

ICC/IF image of ab109486 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109486 at 5μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Lab

Western blot - Anti-WAC antibody (AB109486)

The predicted molecular weight of WAC is71 kDa (SwissProt), however we expect to observe a banding pattern around 85 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab109486 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-WAC antibody (ab109486) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 25 µg

Lane 3:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 25 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 71 kDa

Observed band size: 85 kDa

true

Exposure time: 20min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1) (PubMed : 21329877). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription (PubMed : 21329877). Regulates the cell-cycle checkpoint activation in response to DNA damage (PubMed : 21329877). Positive regulator of amino acid starvation-induced autophagy (PubMed : 22354037). Also acts as a negative regulator of basal autophagy (PubMed : 26812014). Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation (PubMed : 26812014). May negatively regulate the ubiquitin proteasome pathway (PubMed : 21329877).

See full target information WAC

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406759 PubMed39840526

2025

Nuclear Condensates of WW Domain-Containing Adaptor With Coiled-Coil Regulate Mitophagy via Alternative Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahe Wang,Yi Fan,Guowen Luo,Liang Xiong,Lijie Wang,Zhuoxuan Wu,Jiayi Wang,Zhengying Peng,Clifford J Rosen,Kefeng Lu,Junjun Jing,Quan Yuan,Zhenwei Zhang,Chenchen Zhou

Biology 12: PubMed37106788

2023

Structure-Function of the Human WAC Protein in GABAergic Neurons: Towards an Understanding of Autosomal Dominant DeSanto-Shinawi Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah C Rudolph,April M Stafford,Hye-Eun Hwang,Cheol-Hee Kim,Jeremy W Prokop,Daniel Vogt

Molecular therapy. Nucleic acids 31:568-585 PubMed36910712

2023

BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Jinteng,Xu Peitao,Yu Wenhui,Ye Guiwen,Ye Feng,Xu Xiaojun,Su Zepeng,Lin Jiajie,Che Yunshu,Zhang Zhaoqiang,Zeng Yipeng,Li Zhikun,Feng Pei,Cao Qian,Li Dateng,Xie Zhongyu,Wu Yanfeng,Shen Huiyong

Cancer discovery 10:1566-1589 PubMed32703770

2020

Intraductal Transplantation Models of Human Pancreatic Ductal Adenocarcinoma Reveal Progressive Transition of Molecular Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Miyabayashi,Lindsey A Baker,Astrid Deschênes,Benno Traub,Giuseppina Caligiuri,Dennis Plenker,Brinda Alagesan,Pascal Belleau,Siran Li,Jude Kendall,Gun Ho Jang,Risa Karakida Kawaguchi,Tim D D Somerville,Hervé Tiriac,Chang-Il Hwang,Richard A Burkhart,Nicholas J Roberts,Laura D Wood,Ralph H Hruban,Jesse Gillis,Alexander Krasnitz,Christopher R Vakoc,Michael Wigler,Faiyaz Notta,Steven Gallinger,Youngkyu Park,David A Tuveson

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WAC antibody

Immunocytochemistry/ Immunofluorescence - Anti-WAC antibody (AB109486)

Western blot - Anti-WAC antibody (AB109486)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise