AB202025

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4]

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Mouse Monoclonal WASP/Wiskott-Aldrich syndrome protein antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human WAS aa 50-200.

대체 명칭 보기

IMD2, WAS, Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Immunohistochemical analysis of paraffin embedded Human ovarian cancer tissues labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution, followed by DAB staining.

Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Flow cytometric analysis of HeLa cells labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution (green) and negative control (red).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Immunohistochemical analysis of paraffin embedded Human colon cancer tissues labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution, followed by DAB staining.

Supplier Data

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

All lanes:

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025) at 1/500 dilution

Lane 1:

Non transfected HEK293 cell lysate at 1 µg

Lane 2:

Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170)-hIgGFc transfected HEK293 cell lysate at 1 µg

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Expected MW is 39 kDa

All lanes:

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025) at 1/500 dilution

All lanes:

Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170) recombinant protein at 1 µg

Predicted band size: 53 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

7B10E4

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human WAS aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P42768

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from tissue culture supernatant.

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed : 12235133, PubMed : 12769847, PubMed : 16275905). Important for efficient actin polymerization (PubMed : 12235133, PubMed : 16275905, PubMed : 8625410). Possible regulator of lymphocyte and platelet function (PubMed : 9405671). Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed : 18650809). In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 20574068). Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information WAS

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental hematology & oncology 13:109 PubMed39497152

2024

Nuclear porcupine mediates XRCC6/Ku70 S-palmitoylation in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Mingming Xiao,Yaqi Mo,Jinlu Ma,Yamei Han,Qing Li,Qinghua Zeng,Rebecca J Boohaker,Joshua Fried,Yonghe Li,Han Wang,Bo Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (AB202025)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise