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AB224221

Anti-WDHD1 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal WDHD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDHD1 aa 200-350.

대체 명칭 보기

AND1, WDHD1, WD repeat and HMG-box DNA-binding protein 1, Acidic nucleoplasmic DNA-binding protein 1, And-1

4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for WDHD1 (green) using ab224221 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

Paraffin-embedded human tonsil tissue stained for WDHD1 using ab224221 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-WDHD1 antibody (AB224221)
  • WB

Supplier Data

Western blot - Anti-WDHD1 antibody (AB224221)

All lanes:

Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 126 kDa

true

Western blot - Anti-WDHD1 antibody (AB224221)
  • WB

Supplier Data

Western blot - Anti-WDHD1 antibody (AB224221)

All lanes:

Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 126 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human WDHD1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

O75717

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Core replisome component that acts as a replication initiation factor. Binds directly to the CMG complex and functions as a hub to recruit additional proteins to the replication fork.
See full target information WDHD1

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2383886 PubMed39072709

2024

Design, synthesis, and evaluation of novel stilbene derivatives that degrade acidic nucleoplasmic DNA-binding protein 1 (And1) and synergize with PARP1 inhibitor in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leyuan Chen,Zhonghao Ren,Yunze Zhang,Wenbin Hou,Yiliang Li

FEBS open bio 13:102-117 PubMed36345604

2022

WDHD1 is over-expressed in nasopharyngeal carcinoma and may control the expression of ITGAV.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Yun Wu,Yi-Tong Niu,Su-Ning Huang,Yu-Min Tan,Zhen-Dong Yang,Ye-Ying Fang,Li Jiang,Ting-Ting Zhang,Xiao-Fen Zeng,Yun-Xi Peng,Miao Mo,Cai-Xing Lin,Zhu-Xin Wei

Nature communications 13:6090 PubMed36241664

2022

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri Lukas

Science (New York, N.Y.) 369: PubMed32973005

2020

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Sue Im Sim,Alban Ordureau,Lianjie Wei,J Wade Harper,Sichen Shao,Eunyong Park
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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