AB252878

Anti-WDR12 antibody [1B8]

Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rat Recombinant Monoclonal WDR12 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

Ribosome biogenesis protein WDR12, WD repeat-containing protein 12, WDR12

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling WDR12 with ab252878 at 12.35 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling WDR12 with ab252878 at 12.35 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HEK-293 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Lab

Western blot - Anti-WDR12 antibody [1B8] (AB252878)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1/3 : 3 seconds; Lane 2 : 15 seconds; Lane 4 : 1 second.

All lanes:

Western blot - Anti-WDR12 antibody [1B8] (ab252878) at 1.235 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

false

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

1B8

Isotype

IgG1

Light chain 타입

lambda

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.235 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "12.35 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1.235 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1.235 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

See full target information WDR12

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5294-5313 PubMed35672148

2022

Microglial mTOR Activation Upregulates Trem2 and Enhances β-Amyloid Plaque Clearance in the Alzheimer's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Shi,Cheng Chang,Afaf Saliba,Manzoor A Bhat

Frontiers in oncology 11:751792 PubMed34868955

2021

Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Lanjuan Mi,Qinghui Qi,Haowen Ran,Lishu Chen,Da Li,Dake Xiao,Jiaqi Wu,Yan Cai,Songyang Zhang,Yuanyuan Li,Bohan Li,Jiong Xie,Haohao Huang,Tao Li,Tao Zhou,Ailing Li,Ji Qi,Fangye Li,Jianghong Man

The Journal of cell biology 170:367-78 PubMed16043514

2005

Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hölzel,Michaela Rohrmoser,Martin Schlee,Thomas Grimm,Thomas Harasim,Anastassia Malamoussi,Anita Gruber-Eber,Elisabeth Kremmer,Wolfgang Hiddemann,Georg W Bornkamm,Dirk Eick

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDR12 antibody [1B8]

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Western blot - Anti-WDR12 antibody [1B8] (AB252878)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise