AB85962

Anti-WDR26 antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal WDR26 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human WDR26.

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Western blot - Anti-WDR26 antibody (AB85962)

All lanes:

Western blot - Anti-WDR26 antibody (ab85962) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 72 kDa

Observed band size: 220 kDa,50 kDa,80 kDa,90 kDa

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Exposure time: 3min

CiteAb

Western blot - Anti-WDR26 antibody (AB85962)

WDR26 western blot using anti-WDR26 antibody ab85962. Publication image and figure legend from Maitland, M. E. R., Onea, G., et al., 2019, Sci Rep, PubMed 31285494.

ab85962 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab85962 please see the product overview.

Characterization of the CTLH complex. (a) Schematic representation of the CTLH complex. The model is adapted from the yeast Gid complex26. Note that the position of muskelin in the complex has not been formally defined. (b) Subunits of the CTLH complex are present in RanBPM immunocomplexes. HEK293 whole cell extracts were incubated with a RanBPM antibody and immunoprecipitated. Immunoprecipitates were analyzed by Western blot with the indicated antibodies. IgG was used as a negative control. (c) WDR26 associates with the CTLH complex. Whole cell extracts were prepared from HeLa cells untransfected (−) or transfected with FLAG-tagged WDR26 (+). FLAG-WDR26 was immunoprecipitated with a FLAG antibody and immunoprecipitates were analyzed by Western blot with the indicated antibodies. (d) GID4 associates with CTLH complex. Whole cell extracts were prepared from HEK293 cells untransfected (−) or transfected with HA tagged GID4 (+). HA-GID4 was immunoprecipitated with an HA antibody and immunoprecipitates were analyzed by Western blot with the indicated antibodies.

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human WDR26. Database link Q9H7D7

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Reactivity 정보

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab85962 was affinity purified using an epitope specific to WDR26 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

WDR26 also known as WD repeat domain 26 is part of the WD repeat protein family which is important in protein-protein interactions. It weighs roughly 109 kDa. WDR26 expresses in various tissues with significant levels observed in the brain and testis. The protein itself has a molecular architecture enriched with WD repeats which facilitates its involvement in cellular processes by mediating complex formation and signal transduction.

Biological function summary

In the cellular environment WDR26 engages in regulating intracellular signaling pathways. It is a component of the STRIPAK complex which includes several proteins playing roles in synaptic and cellular signaling. The involvement of WDR26 in the STRIPAK complex as a scaffold aids in the correct assembly and localization of the complex impacting cellular processes such as apoptosis and proliferation.

Pathways

WDR26 integrates into key signaling pathways like the MAPK signaling pathway. It interacts with other proteins such as STRN3 and MOB3 to modulate signals that affect gene expression growth and cell differentiation. The MAPK pathway in particular facilitates responses to growth factors stress signals and mitogens illustrating the protein's versatility in signal transduction.

Defects or dysregulation of WDR26 relate to intellectual disabilities and certain neurodevelopmental disorders. Research indicates that changes in WDR26 expression can disturb normal brain development and function often connecting with other proteins like MEK1 which also participates in neurodevelopment. These connections link WDR26 closely to neurological functions and highlight its potential as a target for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G-beta-like protein involved in cell signal transduction (PubMed : 15378603, PubMed : 19446606, PubMed : 22065575, PubMed : 23625927, PubMed : 26895380, PubMed : 27098453). Acts as a negative regulator in MAPK signaling pathway (PubMed : 15378603). Functions as a scaffolding protein to promote G beta : gamma-mediated PLCB2 plasma membrane translocation and subsequent activation in leukocytes (PubMed : 22065575, PubMed : 23625927). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed : 29911972). Acts as a negative regulator of the canonical Wnt signaling pathway through preventing ubiquitination of beta-catenin CTNNB1 by the beta-catenin destruction complex, thus negatively regulating CTNNB1 degradation (PubMed : 27098453). Serves as a scaffold to coordinate PI3K/AKT pathway-driven cell growth and migration (PubMed : 26895380). Protects cells from oxidative stress-induced apoptosis via the down-regulation of AP-1 transcriptional activity as well as by inhibiting cytochrome c release from mitochondria (PubMed : 19446606). Also protects cells by promoting hypoxia-mediated autophagy and mitophagy (By similarity).

See full target information WDR26

대체 명칭 보기

CDW2, MIP2, PRO0852, WDR26, WD repeat-containing protein 26, CUL4- and DDB1-associated WDR protein 2, Myocardial ischemic preconditioning up-regulated protein 2

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta neuropathologica 149:44 PubMed40317322

2025

The interactome of tau phosphorylated at T217 in Alzheimer's disease human brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Kavanagh,Manon Thierry,Kaleah Balcomb,Jackeline Ponce,Evgeny Kanshin,Alexander Tapia-Sealey,Glenda Halliday,Beatrix Ueberheide,Thomas Wisniewski,Eleanor Drummond

Communications biology 7:1668 PubMed39702571

2024

Interplay between β-propeller subunits WDR26 and muskelin regulates the CTLH E3 ligase supramolecular complex.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew E R Maitland,Gabriel Onea,Dominic D G Owens,Brianna C Gonga-Cavé,Xu Wang,Cheryl H Arrowsmith,Dalia Barsyte-Lovejoy,Gilles A Lajoie,Caroline Schild-Poulter

Molecular cell 84:1948-1963.e11 PubMed38759627

2024

Non-canonical substrate recognition by the human WDR26-CTLH E3 ligase regulates prodrug metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik V Gottemukkala,Jakub Chrustowicz,Dawafuti Sherpa,Sara Sepic,Duc Tung Vu,Özge Karayel,Eleftheria C Papadopoulou,Annette Gross,Kenji Schorpp,Susanne von Gronau,Kamyar Hadian,Peter J Murray,Matthias Mann,Brenda A Schulman,Arno F Alpi

Scientific reports 10:7138 PubMed32346083

2020

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

Applications

Unspecified application

Species

Unspecified reactive species

Shimaa H A Soliman,Aaron E Stark,Miranda L Gardner,Sean W Harshman,Chelssie C Breece,Foued Amari,Arturo Orlacchio,Min Chen,Anna Tessari,Jennifer A Martin,Rosa Visone,Michael A Freitas,Krista M D La Perle,Dario Palmieri,Vincenzo Coppola

Scientific reports 9:9864 PubMed31285494

2019

The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew E R Maitland,Gabriel Onea,Christopher A Chiasson,Xu Wang,Jun Ma,Sarah E Moor,Kathryn R Barber,Gilles A Lajoie,Gary S Shaw,Caroline Schild-Poulter

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDR26 antibody

Western blot - Anti-WDR26 antibody (AB85962)

Western blot - Anti-WDR26 antibody (AB85962)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise