AB31962

Anti-Wnt11 antibody

Name: Anti-Wnt11 antibody
Brand: Abcam Limited
SKU: AB31962
Rating: 4 (8 reviews)

(8 리뷰들)

(22 제품이 사용된 논문 )

Rabbit Polyclonal WNT11 antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 22 publications.

대체 명칭 보기

Protein Wnt-11, WNT11

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

ab31962 staining Wnt11 in HaCat cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31962 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Supplier Data

Western blot - Anti-Wnt11 antibody (AB31962)

Blocking Buffer : 2% BSA

All lanes:

Western blot - Anti-Wnt11 antibody (ab31962) at 1 µg/mL

All lanes:

Human Wnt11 Recombinant Protein at 0.01 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.This antibody detects recombinant human Wnt11 by Western Blotting. We have been unable to detect endogenous protein in cell lines with this antibody, possibly due to the low level of endogenous protein in the samples tested.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

See full target information WNT11

style

left-column

제품이 사용된 논문 (22)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bone research 13:17 PubMed39865089

2025

Skeletal progenitor LRP1 deficiency causes severe and persistent skeletal defects with Wnt pathway dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Alhashmi,Abdulrahman M E Gremida,Santosh K Maharana,Marco Antonaci,Amy Kerr,Shijian Fu,Sharna Lunn,David A Turner,Noor A Al-Maslamani,Ke Liu,Maria M Meschis,Hazel Sutherland,Peter Wilson,Peter Clegg,Grant N Wheeler,Robert J van 't Hof,George Bou-Gharios,Kazuhiro Yamamoto

Cell death & disease 15:84 PubMed38267432

2024

Mechanical stiffness promotes skin fibrosis via Piezo1-Wnt2/Wnt11-CCL24 positive feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahao He,Xinwei Cheng,Bin Fang,Shengzhou Shan,Qingfeng Li

Scientific reports 13:13331 PubMed37587207

2023

Hedyotis diffusa-Sculellaria barbata (HD-SB) suppresses the progression of colorectal cancer cells via the hsa_circ_0039933/hsa-miR-204-5p/wnt11 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Danye Zhu,Shanmin Yuan,Cong Chen

The European respiratory journal : PubMed32859680

2020

Wnt5a and Wnt11 as acute respiratory distress syndrome biomarkers for SARS-CoV-2 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Young Choi,Hee Ho Park,Hyelim Kim,Hong Nam Kim,Inyoung Kim,Soyoung Jeon,Wantae Kim,Jong-Sup Bae,Wonhwa Lee

The Journal of clinical investigation 130:4152-4166 PubMed32597829

2020

Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Wakako Kawarazaki,Risuke Mizuno,Mitsuhiro Nishimoto,Nobuhiro Ayuzawa,Daigoro Hirohama,Kohei Ueda,Fumiko Kawakami-Mori,Shigeyoshi Oba,Takeshi Marumo,Toshiro Fujita

Molecular medicine reports 22:1839-1846 PubMed32582982

2020

Tanshinone IIA promotes cardiac differentiation and improves cell motility by modulating the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Xiuyan Wang,Chenxing Fan,Chunxia Wu,Shizheng Li,Hua Liu

Cell reports 30:771-782.e6 PubMed31968252

2020

Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Ning Yin,Xue Wang,Andras Khoor,Vaishnavi Sambandam,Anwesha B Ghosh,Zoe A Fields,Nicole R Murray,Verline Justilien,Alan P Fields

Experimental and therapeutic medicine 18:4631-4636 PubMed31798700

2019

Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Chen,Shunen Xu,Wei Li,Lirong Wu,Long Wang,Yongkang Li,Wei Zhou

Journal of cellular physiology 234:18639-18652 PubMed30924167

2019

Protein complexes associated with β-catenin differentially influence the differentiation profile of neonatal and adult CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gerson N Hernández-Acevedo,Oscar H López-Portales,Darely Y Gutiérrez-Reyna,Erick Cuevas-Fernández,Linda A Kempis-Calanis,Rosario G Labastida-Conde,Oscar B Aguilar-Luviano,Oscar Ramírez-Pliego,Salvatore Spicuglia,Bárbara Lino-Alfaro,Alicia Chagolla-López,Luis E González-de la Vara,María Angélica Santana

Cellular physiology and biochemistry : internation 49:2254-2263 PubMed30257243

2018

Hyperglycemia Altered the Fate of Cardiac Stem Cells to Adipogenesis through Inhibiting the β-Catenin/TCF-4 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Zhang,Ke Meng,Yujie Pu,Chao Wang,Yingying Chen,Linlin Wang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt11 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

Western blot - Anti-Wnt11 antibody (AB31962)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (22)

Product promise