AB96730

Anti-Wnt11 antibody

Name: Anti-Wnt11 antibody
Brand: Abcam Limited
SKU: AB96730
Rating: 1 (1 reviews)

(1 리뷰)

(8 제품이 사용된 논문 )

Rabbit Polyclonal WNT11 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment, Rat, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT11 aa 1-300.

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Paraffin-embedded lung SCC xenograft satined Wnt11 using ab96730 at 1/100 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) using ab96730 at 1/200 dilution. Lower image merged with DNA probe.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunofluorescence analysis of ice-cold MeOH-fixed (5 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) at cytoplasm using ab96730 at 1/500 dilution. Blue : Hoechst 33342 staining. Scale bar = 10 μm.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Paraffin-embedded mouse colon tissue stained Wnt11 using ab96730 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

COLO 205 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

10% SDS-PAGE

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

COLO 205 (human colon adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

Mouse heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/5000 dilution

All lanes:

Rat heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

true

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

10% SDS-PAGE

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

Mouse white adipose tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WNT11 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O96014

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

See full target information WNT11

대체 명칭 보기

Protein Wnt-11, WNT11

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Orphanet journal of rare diseases 20:289 PubMed40483479

2025

A de novo SALL4 mutation causes unilateral renal agenesis by misregulating genes involved in kidney development.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zhao,Yali Fan,Jieyan Li,Lin Li,Chenghong Yin

Chemistry & biodiversity 20:e202200768 PubMed36694378

2023

Possible Mechanism of Dysphania ambrosioides (L.) Mosyakin & Clemants Seed Extract Suppresses the Migration and Invasion of Human Hepatocellular Carcinoma Cells SMMC-7721.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Huang,Junmei Hao,Jintao Nie,Ruihua Qian,Haiying Li,Jiayuan Zhao,Yanan Wang

Bone & joint research 10:558-570 PubMed34465146

2021

Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Li,Zhi Peng,You Zhou,Yongyue Su,Pengfei Bu,Xuhan Meng,Bo Li,Yongqing Xu

The Kaohsiung journal of medical sciences 37:768-775 PubMed34002485

2021

Long non-coding RNA-H19 promotes ovarian cancer cell proliferation and migration via the microRNA-140/Wnt1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Wang,Wei-Jiao Gao

American journal of physiology. Cell physiology 317:C189-C199 PubMed31042421

2019

MicroRNA-140 inhibits skeletal muscle glycolysis and atrophy in endotoxin-induced sepsis in mice via the WNT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Tian-Mei Li,Xue-Ru Liu,Yi-Ping Bai,Jie Li,Ni Tang,Xiao-Bin Wang

Frontiers in oncology 7:92 PubMed28589081

2017

Inhibitory Effect of 1,8-Cineol on β-Catenin Regulation, WNT11 Expression, and Cellular Progression in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Roettger,Karl-Ludwig Bruchhage,Maren Drenckhan,Kirsten Ploetze-Martin,Ralph Pries,Barbara Wollenberg

Stem cells (Dayton, Ohio) 32:1649-60 PubMed24496952

2014

Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Emily C Keats,James M Dominguez,Maria B Grant,Zia A Khan

Breast cancer research : BCR 14:R98 PubMed22748014

2012

Lack of transforming growth factor-β signaling promotes collective cancer cell invasion through tumor-stromal crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren A Matise,Trenis D Palmer,William J Ashby,Abudi Nashabi,Anna Chytil,Mary Aakre,Michael W Pickup,Agnieszka E Gorska,Andries Zijlstra,Harold L Moses

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (8)

Product promise