JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB281094

Anti-Wnt16 antibody [EPR22889-129] - BSA and Azide free (Detector)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal WNT16 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
1 이미지
Sandwich ELISA - Anti-Wnt16 antibody [EPR22889-129] - BSA and Azide free (Detector) (AB281094)
  • sELISA

Lab

Sandwich ELISA - Anti-Wnt16 antibody [EPR22889-129] - BSA and Azide free (Detector) (AB281094)

Sandwich ELISA of ab253684 with the capture antibody ab281244 dilution at 2 µg/mL and detector antibody ab281094 dilution at 0.5 µg/mL.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22889-129

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
Sample Prep & Detection Kits

AB269903

Gold-Maleimide Conjugation Kit (10nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (10nm, 20 OD) (AB269903)

  • 0
  • 0
Primary Antibodies

AB281017

Anti-Wnt7a antibody [EPR23471-215] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Wnt7a antibody [EPR23471-215] - BSA and Azide free (Detector) (AB281017)

  • 0
  • 0
Primary Antibodies

AB243020

Anti-IL-12B antibody [EPR20096-81] - BSA and Azide free (Capture/Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-12B antibody [EPR20096-81] - BSA and Azide free (Capture/Detector) (AB243020)

  • 0
  • 0
Primary Antibodies

AB303711

Anti-VEGFC antibody [EPR22854-182] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-VEGFC antibody [EPR22854-182] - BSA and Azide free (Detector) (AB303711)

  • 0
  • 0
Primary Antibodies

AB309581

Anti-SMOC1 antibody [EPR26922-76] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-SMOC1 antibody [EPR26922-76] - BSA and Azide free (Detector) (AB309581)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab281094 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human WNT16. The recommended pair for sandwich ELISA is:
Capture: ab281244, Human WNT16 Capture Antibody (unconjugated)
Detector: ab281094, Human WNT16 Detector Antibody (unconjugated)
The reference range value is 19.53-2500 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt16 also known as wingless-type MMTV integration site family member 16 is a protein with a mass of approximately 43 kDa. It belongs to the Wnt protein family which comprises secreted signaling proteins. Wnt16 facilitates the transfer of signals between cells influencing various cellular processes. This protein shows expression in several tissues such as bone cartilage and hematopoietic tissues playing roles in maintaining their functions.
Biological function summary

Wnt16 influences bone development and maintenance by enhancing osteoblast proliferation and differentiation. It functions as part of a complex signaling cascade interacting with receptors on cell surfaces to initiate intracellular signaling pathways. One of its primary roles is in balancing bone formation and resorption ensuring skeletal integrity. Furthermore Wnt16 contributes to cartilage homeostasis preventing degenerative changes that could lead to diseases like osteoarthritis.

Pathways

Wnt16 is part of significant signaling pathways like the canonical Wnt/β-catenin pathway as well as the non-canonical Wnt pathways. In the canonical pathway Wnt16 binds to Frizzled receptors and LRP5/6 co-receptors facilitating the stabilization and translocation of β-catenin into the nucleus affecting gene transcription. In this context Wnt16 relates closely to other Wnt proteins like Wnt3a influencing diverse cellular outcomes such as proliferation and differentiation.

Wnt16 has an impact on conditions like osteoporosis and osteoarthritis. In osteoporosis the reduction in Wnt16 expression correlates with decreased bone density and increased risk of fractures. Through its interaction with bone-forming proteins like Osteocalcin Wnt16 regulates bone mass and strength. Additionally in osteoarthritis Wnt16 downregulation leads to increased cartilage degradation linking it to proteins like MMP-13 which is involved in cartilage breakdown.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
See full target information Protein Wnt-16

대체 명칭 보기

Protein Wnt-16, WNT16
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com