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AB313859

Anti-Wnt16 antibody [HL1498] - BSA and Azide free

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Rabbit Recombinant Monoclonal WNT16 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein Wnt-16.

대체 명칭 보기

Protein Wnt-16, WNT16

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Wnt16 antibody [HL1498] - BSA and Azide free (AB313859)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt16 antibody [HL1498] - BSA and Azide free (AB313859)

Immunofluorescent analysis of mock and Wnt16 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Wnt16 with ab313859 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1498

Isotype

IgG

Carrier free

Yes

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Protein Wnt-16.

Q9UBV4

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보완 제품

Primary Antibodies

AB106522

Anti-Wnt10a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)

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  • 1
Primary Antibodies

AB316738

Anti-Wnt11 antibody [EPR25633-141]

20ul selling size|Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] (AB316738)

  • 0
  • 0
Primary Antibodies

AB274321

Anti-Wnt7a antibody [EPR23471-125]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] (AB274321)

  • 0
  • 0
Primary Antibodies

AB50030

Anti-Wnt6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt6 antibody (AB50030)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt16 also known as wingless-type MMTV integration site family member 16 is a protein with a mass of approximately 43 kDa. It belongs to the Wnt protein family which comprises secreted signaling proteins. Wnt16 facilitates the transfer of signals between cells influencing various cellular processes. This protein shows expression in several tissues such as bone cartilage and hematopoietic tissues playing roles in maintaining their functions.
Biological function summary

Wnt16 influences bone development and maintenance by enhancing osteoblast proliferation and differentiation. It functions as part of a complex signaling cascade interacting with receptors on cell surfaces to initiate intracellular signaling pathways. One of its primary roles is in balancing bone formation and resorption ensuring skeletal integrity. Furthermore Wnt16 contributes to cartilage homeostasis preventing degenerative changes that could lead to diseases like osteoarthritis.

Pathways

Wnt16 is part of significant signaling pathways like the canonical Wnt/β-catenin pathway as well as the non-canonical Wnt pathways. In the canonical pathway Wnt16 binds to Frizzled receptors and LRP5/6 co-receptors facilitating the stabilization and translocation of β-catenin into the nucleus affecting gene transcription. In this context Wnt16 relates closely to other Wnt proteins like Wnt3a influencing diverse cellular outcomes such as proliferation and differentiation.

Wnt16 has an impact on conditions like osteoporosis and osteoarthritis. In osteoporosis the reduction in Wnt16 expression correlates with decreased bone density and increased risk of fractures. Through its interaction with bone-forming proteins like Osteocalcin Wnt16 regulates bone mass and strength. Additionally in osteoarthritis Wnt16 downregulation leads to increased cartilage degradation linking it to proteins like MMP-13 which is involved in cartilage breakdown.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
See full target information Protein Wnt-16
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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