AB317122

Anti-Wnt2 antibody [HL1999]

Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Rabbit Recombinant Monoclonal Wnt2/IRP antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Mouse, Rat, Zebrafish, Recombinant full length protein samples. Immunogen corresponding to Recombinant Fragment Protein within Human WNT2.

대체 명칭 보기

INT1L1, IRP, WNT2, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein

3 이미지

Supplier Data

Western blot - Anti-Wnt2 antibody [HL1999] (AB317122)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317122 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt2 antibody [HL1999] (ab317122) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Wnt2 antibody [HL1999] (AB317122)

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317122 diluted at 1 : 10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt2 antibody [HL1999] (ab317122) at 1/10000 dilution

All lanes:

Mouse brain tissue extract

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunocytochemistry/ Immunofluorescence - Anti-Wnt2 antibody [HL1999] (AB317122)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt2 antibody [HL1999] (AB317122)

Immunofluorescence analysis of Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling Wnt2 protein using ab317122 at a 1/500 dilution (Green).
DAPI was used for nuclear staining (Blue).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1999

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Zebrafish

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human WNT2.

P09544

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/15000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt2 also known as Wingless-type MMTV integration site family member 2 or IRP is a protein around 41 kDa in mass. It is mechanically involved in signaling processes playing an important role in cell-to-cell communication. Wnt2 is expressed in various tissues including the heart lung and spleen and it participates in embryonic development and organogenesis. This protein belongs to the Wnt family which is known for modulating developmental processes and signaling pathways.

Biological function summary

With respect to the Wnt/β-catenin signaling pathway Wnt2 operates as a ligand that binds to Frizzled receptors on the cell surface. This interaction leads to the stabilization of β-catenin in the cytoplasm impacting gene transcription. Wnt2 does not function alone; it forms complexes with other proteins to efficiently propagate signals that regulate cell fate decisions. It is important in maintaining homeostasis and regeneration processes in adults.

Pathways

Within the Wnt/β-catenin pathway Wnt2 activation regulates important genes involved in differentiation and proliferation. This protein interacts with key molecules like Axin and GSK-3β facilitating the stabilization of β-catenin and subsequent gene transcription. Another pathway involving Wnt2 is the planar cell polarity pathway where it influences cytoskeletal arrangements and directional cell movement through its interaction with Dishevelled proteins.

Research links Wnt2 to cancer progression and fibrotic diseases. Aberrant Wnt2 signaling promotes tumorigenesis through deregulated cell proliferation and survival particularly in gastric and colorectal cancers. In fibrosis overactive Wnt2 signaling contributes to excessive extracellular matrix production with Transforming Growth Factor-β (TGF-β) often collaborating in these processes to exacerbate disease progression. Understanding how Wnt2 interacts with these proteins offers insight into potential therapeutic targets.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20018874). Functions as a upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).

See full target information WNT2

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt2 antibody [HL1999]

Western blot - Anti-Wnt2 antibody [HL1999] (AB317122)

Western blot - Anti-Wnt2 antibody [HL1999] (AB317122)

Immunocytochemistry/ Immunofluorescence - Anti-Wnt2 antibody [HL1999] (AB317122)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise