AB240167

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal WNT3A antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Protein Wnt-3a, WNT3A

1 이미지

Supplier Data

Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free (AB240167)

This data was developed using ab172612, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (<a href='/ko/products/primary-antibodies/wnt3-and-wnt3a-antibody-epr49202-ab172612'>ab172612</a>) at 1/10000 dilution

Lane 1:

CHO cell lysate, non-transfected at 10 µg

Lane 2:

CHO cell lysate, transfected with Human Wnt3a at 10 µg

Predicted band size: 39 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4920(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for both Wnt3 and Wnt3a based on the fact that the immunogen sequence is 100% identical for both.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab240167 is the carrier-free version of ab172612.

This antibody was developed as part of a collaboration with Karl Willert’s lab from the University of California at San Diego.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt3 and Wnt3a both members of the Wnt family are secreted glycoproteins involved in several signaling pathways. Commonly known as Wnt3 and Wnt3a for simplicity they have an approximate mass of around 40 kDa. These proteins exhibit expression during development stages and in various adult tissues. They function mechanistically by binding to Frizzled family receptors activating downstream signaling cascades. Wnt3 is predominantly expressed in the central nervous system while Wnt3a shows wide expression across mesoderm and ectoderm tissues.

Biological function summary

Wnt3 and Wnt3a are key players in cell proliferation differentiation and migration. These proteins are part of the Wnt signaling complex which influences gene expression important for embryonic development and tissue homeostasis. The Wnt3 and Wnt3a promote stem cell maintenance and expansion by regulating transcriptional activators and repressors. Their role extends to maintaining cellular architecture through interaction with cytoskeletal elements.

Pathways

Wnt3 and Wnt3a are integral components of the canonical Wnt/β-catenin pathway and the Wnt/Ca2+ non-canonical pathway. Through these pathways they impact various biological processes essential to cellular growth and differentiation. Wnt3a often interacts with β-catenin a well-known mediator that influences gene expression by entering the nucleus. Both Wnts regulate pathways that include Dishevelled (Dvl) proteins highlighting their interconnectedness in cell signaling networks.

Alterations in Wnt3 and Wnt3a signaling contribute to cancer and neurodegenerative diseases. Abnormal Wnt/β-catenin signaling links to colorectal cancer where excessive β-catenin activity supports tumor growth. Moreover disrupted Wnt signaling can impact Alzheimer's disease pathogenesis through interactions with amyloid precursor protein and tau. These connections highlight how Wnt3 and Wnt3a influence disease states impacting therapeutic strategies targeting Wnt pathway components.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750

2023

Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mengzhu,Zhang Yujie,Song Yafang,Guo Jing,Wang Yuhang,Xin Chen,G U Dongmei,Sun Jianhua,Pei Lixia

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free

Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free (AB240167)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise