AB50341

Anti-Wnt3 antibody

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(5 제품이 사용된 논문 )

Goat Polyclonal WNT3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human WNT3 aa 300-350.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB50341)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB50341)

ab50341 (2μg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.

Unknown

Western blot - Anti-Wnt3 antibody (AB50341)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Wnt3 antibody (ab50341) at 1 µg/mL

All lanes:

Human Brain lysate (35µg protein in RIPA buffer).

Predicted band size: 40 kDa

Observed band size: 17 kDa,38 kDa

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CiteAb

Western blot - Anti-Wnt3 antibody (AB50341)

Wnt3 western blot using anti-Wnt3 antibody ab50341. Publication image and figure legend from Tripathy, A., Thakurela, S., et al., 2018, PLoS One, PubMed 30513115.

ab50341 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50341 please see the product overview.

The higher activity of Wnt and Hh pathway in promotion-group correlates with moderately-differentiated/grade-II HCC.Enhanced Wnt and Hh signaling correlates with moderately differentiated HCC. (A) H and E stained liver sections of control and promotion group (group2) animals showing moderately differentiated, thick trabecular pattern, followed by photomicraphs showing IHC staining of Wnt and Hh pathway molecules at TE and TL phases of promotion stage. The corresponding IHC staining intensity graph is shown in the side panel of figures.Y-axis represents labeling index (%) visual score in each intensity graph. (B) Increasing mRNA expression level of Wnt and Hh pathway molecules of control and group2 animals at TE and TL phases of promotion stage. (C) The figure represents fold increase in relative mRNA expression, calculated with respect to GAPDH for Shh, Ptch1, Gli1, Wnt3 β-catenin in control and group2 animals at TE and TL phases of promotion stage (D) Analysis of Wnt and Hh pathway molecules in rat tissue lysates at TE and TL phases of promotion stage by ELISA. Data presented are representative of three independent experiments performed in triplicates and expressed as Mean±S.D. ** and *** differs significantly at p < 0.005 and <0.0005.

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주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human WNT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P56703

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt3 also known as Wingless-related integration site 3 or Proto-oncogene Wnt-3 plays an important mechanical role in cellular signaling. It is a protein with a mass of approximately 38 kDa. Wnt3 is expressed richly across various tissues including brain heart and skeletal muscle. This protein is instrumental in initiating signaling cascades that influence cell behavior during development.

Biological function summary

Wnt3 functions in the Wnt signaling pathway to regulate gene transcription cellular polarity and cell fate determinations. As a member of the Wnt protein family Wnt3 contributes to a protein complex on the cell surface interacting with cell surface receptors such as frizzled receptors. These interactions trigger intracellular signaling pathways that promote developmental processes and maintain cellular communication networks.

Pathways

Wnt3 is integral to the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. In the canonical pathway interactions with β-catenin lead to gene expression changes critical for cell differentiation and proliferation. In the non-canonical pathway Wnt3 associates with proteins like Dishevelled and phosphoproteins to influence cytoskeletal dynamics and cellular movement. These pathways highlight Wnt3's involvement in critical cellular functions and developmental processes.

Aberrant Wnt3 signaling is linked to various conditions including cancer and osteoporosis. The protein's role in cancer often together with the β-catenin protein reveals connections in tumor growth and metastasis. In osteoporosis dysregulation of Wnt3 impacts bone density and strength as it affects osteoblast function in bone formation. These associations highlight Wnt3's significant involvement in pathological states when its signaling is disrupted.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed : 14872406).

See full target information WNT3

대체 명칭 보기

INT4, WNT3, Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Gut microbes 16:2347722 PubMed38706205

2024

Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Wei Xie,Shang Cai,Hai-Yan Lu,Feng-Ling Tang,Rui-Qiu Zhu,Ye Tian,Ming Li

Cell host & microbe 24:833-846.e6 PubMed30543778

2018

Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Soo Lee,Tae-Young Kim,Yeji Kim,Su-Hyun Lee,Seungil Kim,Sung Wan Kang,Jin-Young Yang,In-Jeoung Baek,Young Hoon Sung,Yun-Yong Park,Sung Wook Hwang,Eunju O,Kwang Soon Kim,Siqing Liu,Nobuhiko Kamada,Nan Gao,Mi-Na Kweon

PloS one 13:e0208194 PubMed30513115

2018

The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Anindita Tripathy,Sudhir Thakurela,Manoj Kumar Sahu,Kanishka Uthanasingh,Manas Behera,Amrendra Kumar Ajay,Ratna Kumari

Journal of cellular biochemistry 119:9316-9326 PubMed30074264

2018

MicroRNA-136 promotes lipopolysaccharide-induced ATDC5 cell injury and inflammatory cytokine expression by targeting myeloid cell leukemia 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Daliang Kong

Cell death and differentiation 26:426-442 PubMed29786076

2018

c-Myc inhibits myoblast differentiation and promotes myoblast proliferation and muscle fibre hypertrophy by regulating the expression of its target genes, miRNAs and lincRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Luo,Jiahui Chen,Limin Li,Xueyi Ren,Tian Cheng,Shiyi Lu,Raman Akinyanju Lawal,Qinghua Nie,Xiquan Zhang,Olivier Hanotte

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB50341)

Western blot - Anti-Wnt3 antibody (AB50341)

Western blot - Anti-Wnt3 antibody (AB50341)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise