AB81614

Anti-Wnt3a antibody [3A6]

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(2 리뷰들)

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(21 제품이 사용된 논문 )

Mouse Monoclonal WNT3A antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WNT3A.

1 이미지

Western blot - Anti-Wnt3a antibody [3A6] (AB81614)

WB

Unknown

Western blot - Anti-Wnt3a antibody [3A6] (AB81614)

All lanes:

Western blot - Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution

Lane 1:

293T cell lysate at 40 µg

Lane 2:

Wnt3a transfected 293T cell lysate at 40 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 39 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3A6

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Recombinant Full Length Protein corresponding to Human WNT3A.

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대체품

Primary Antibodies

AB219412

Anti-Wnt3a antibody [EPR21889]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412)

0
0

Primary Antibodies

AB169175

Anti-Wnt3a antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (AB169175)

0
0

Primary Antibodies

AB28472

Anti-Wnt3a antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (AB28472)

3
6

Primary Antibodies

AB234099

Anti-Wnt3a antibody [EPR21889] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (AB234099)

3
1

보완 제품

Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

5
75

Primary Antibodies

AB109222

Anti-Wnt2/IRP antibody [EPR3101(2)]

RabMAb|Recombinant

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

0
0

Primary Antibodies

AB32391

Anti-GSK3 beta antibody [Y174]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody [Y174] (AB32391)

5
2

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Precipitation Ammonium Sulphate

보관 버퍼

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

대체 명칭 보기

Protein Wnt-3a, WNT3A

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제품이 사용된 논문 (21)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer management and research 17:1989-1997 PubMed41019940

2025

Neuropilin-2 Mediates Radioresistance in Nasopharyngeal Carcinoma via Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Chen,Huimin Fu,Xiaopeng Huang,Zetan Chen

Scientific reports 14:27053 PubMed39511359

2024

Rhomboid-like 2 correlated with TME infiltration inhibits cuproptosis-related genes and drives malignant phenotype in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifei Che,Wenyi Jin,Yaoxi Wu,Haoyong Li,Peiyu Liang

Bioengineered 13:3070-3081 PubMed35100096

2022

High hydrostatic pressure (30 atm) enhances the apoptosis and inhibits the proteoglycan synthesis and extracellular matrix level of human nucleus pulposus cells via promoting the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zongting Shi,Jun He,Jian He,Yuan Xu

Molecular therapy. Nucleic acids 26:1318-1335 PubMed34853730

2021

TGFB3-AS1 promotes Hcy-induced inflammationof macrophages via inhibiting the maturityof miR-144 and upregulating Rap1a.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Yinju Hao,Anning Yang,Lin Xie,Ning Ding,Lingbo Xu,Yanhua Wang,Yong Yang,Yongsheng Bai,Huiping Zhang,Yideng Jiang

Nature communications 12:362 PubMed33441552

2021

Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Lung Wu,Amanda Dicks,Nancy Steward,Ruhang Tang,Dakota B Katz,Yun-Rak Choi,Farshid Guilak

Frontiers in cardiovascular medicine 7:567837 PubMed33330641

2020

Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Ackers,Candice Szymanski,Mitchell J Silver,Ramiro Malgor

Cell cycle (Georgetown, Tex.) 19:3231-3248 PubMed33198579

2020

Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Wen,Chenjiang He,Qin Zhang,Fanglin Zhang,Na Li,Yan Pan,Mengting Deng,Yue Wang,Jianping Li,Jiaxuan Qiu

Molecular oncology 14:462-483 PubMed31785055

2019

Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Tian,Yuanyuan Wu,Yating Yang,Jiaxin Wang,Menglan Niu,Shanjun Gao,Tao Qin,Dengke Bao

Cell communication and signaling : CCS 17:109 PubMed31462314

2019

WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojie Jiang,Miaofeng Zhang,Yanhua Zhang,Weiping Zhou,Tao Zhu,Qing Ruan,Hui Chen,Jie Fang,Fei Zhou,Jihong Sun,Xiaoming Yang

Oncology reports 41:3137-3147 PubMed30896857

2019

microRNA‑485 inhibits the malignant behaviors of retinoblastoma by directly targeting Wnt3a.

Applications

Unspecified application

Species

Unspecified reactive species

Xueman Lyu,Ling Wang,Jia Lu,Hong Zhang,Lina Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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