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AB325532

Anti-Wnt5a antibody [EPR29464-509]

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Rabbit Recombinant Monoclonal WNT5A antibody. Suitable for WB, I-ELISA and reacts with Recombinant fragment - Human, Human, Mouse, Recombinant full length protein - Human samples.
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Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • WB

Lab

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325532 was shown to bind specifically to Wnt5a. Target of interest was observed at 42 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in WNT5A knockout cell line (lane 2) (lane 2, knockout cell line ab264019 / knockout cell lysate ab264515).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Wnt5a antibody [EPR29464-509] (ab325532) at 1/1000 dilution

Lane 1:

WI-38 (human fetal lung fibroblast) whole cell lysate at 80 µg

Lane 2:

Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Lane 3:

WNT5A knockout HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • WB

Lab

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : EL4.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Wnt5a antibody [EPR29464-509] (ab325532) at 1/1000 dilution

Lane 1:

L Wnt-5A (mouse Subcutaneous connective tissue) whole cell lysate at 80 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 180s

Indirect ELISA - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • I-ELISA

Lab

Indirect ELISA - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Indirect ELISA analysis of ab325532 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Wnt5a protein; Human Wnt5b protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • WB

Lab

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Wnt-5b.

In Western blot, Anti-Wnt-5a/b (ab325179) (1 : 1000) (42KDa).

All lanes:

Western blot - Anti-Wnt5a antibody [EPR29464-509] (ab325532) at 1/1000 dilution

Lane 1:

Non-tagged human Wnt-5a fragment at 10 ng

Lane 2:

Non-tagged human Wnt-5b fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa

false

Exposure time: 48s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29464-509

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>For detection of endogenous Wnt5a in WI-38 and HeLa cells we loaded 80ug of total lysate samples.</p>

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보완 제품

Primary Antibodies

AB109222

Anti-Wnt2/IRP antibody [EPR3101(2)]

RabMAb|Recombinant

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

  • 0
  • 0
Primary Antibodies

AB290728

Anti-Frizzled 6 antibody [EPR25319-149]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 6 antibody [EPR25319-149] (AB290728)

  • 0
  • 0
Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

  • 5
  • 75
Primary Antibodies

AB70816

Anti-Wnt10b antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt10b antibody (AB70816)

  • 3
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "250 ng/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed : 15735754). Mediates motility of melanoma cells (PubMed : 17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
See full target information WNT5A

대체 명칭 보기

Protein Wnt-5a, WNT5A
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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