AB238144

Anti-WWOX antibody [EPR23109-138]

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal WWOX antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 2 publications.

대체 명칭 보기

FOR, SDR41C1, WOX1, WWOX, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductase, Short chain dehydrogenase/reductase family 41C member 1

10 이미지

Flow Cytometry (Intracellular) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling WWOX with ab238144 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : MDA-MB-231(PMID : 19188760).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on Leydig cells in human testis (PMID : 16941225) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human breast carcinoma (PMID : 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human cerebrum (PMID : 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Unknown

Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (AB238144)

WWOX was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2 : ab238144 IP in MCF7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab238144 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)

Predicted band size: 47 kDa

Observed band size: 46 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling WWOX with ab238144 at 1/5000 dilution (0.10 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on mouse cerebrum (PMID : 17360458) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Unknown

Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (AB238144)

WWOX was immunoprecipitated from 0.35 mg Mouse testis lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis lysate 10ug

Lane 2 : ab238144 IP in Mouse testis lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab238144 in Mouse testis lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)

Predicted band size: 47 kDa

Observed band size: 46 kDa

false

Unknown

Western blot - Anti-WWOX antibody [EPR23109-138] (AB238144)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1-3 : 37 seconds; Lane 4 : 70 seconds.

All lanes:

Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

Lane 1:

Human testis lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse ovary lysate at 20 µg

Lane 4:

Mouse testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 46 kDa

false

Unknown

Western blot - Anti-WWOX antibody [EPR23109-138] (AB238144)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Negative control : MDA-MB-231 (PMID : 19188760).

All lanes:

Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 46 kDa

false

Supplier Data

Western blot - Anti-WWOX antibody [EPR23109-138] (AB238144)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

true

Exposure time: 37s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23109-138

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein WWOX also known as WW domain-containing oxidoreductase or FOR has a molecular mass of approximately 46 kDa. Mechanically WWOX functions by interacting with several other proteins through its WW domains impacting various cellular processes. It exhibits a wide expression pattern found in numerous tissues including the brain lung and testis. Additionally WWOX localizes mainly in the cytoplasm but also migrates to the nucleus under certain conditions indicating its diverse roles in cellular regulation.

Biological function summary

The protein plays a significant role in cellular processes like apoptosis tumor suppression and steroidogenesis. WWOX participates in various cellular complexes including ones that regulate transcription and signal transduction. Its interaction with the tumor suppressor p53 and other signaling molecules supports its influence on cell cycle regulation and apoptotic pathways. This interaction aids the cell in sensing stress and damage modulating responses to these conditions.

Pathways

WWOX is intimately linked with the Wnt signaling and apoptotic pathways. In the Wnt pathway WWOX regulates cell proliferation and differentiation by interacting with proteins such as Dvl-2 and beta-catenin. In apoptotic pathways WWOX serves a regulatory role by modulating the activity of proteins like JNK and p73 which are important for inducing programmed cell death. These pathways highlight the importance of WWOX in maintaining cellular homeostasis and responding to abnormal cellular activities.

Alterations in WWOX expression have strong ties to cancer and neurodegenerative diseases. Loss or mutation of WWOX associates with breast prostate and lung cancers due to its role in tumor suppression. The protein also connects to neurodegenerative disorders such as Alzheimer's disease with misregulation affecting neuronal survival. In cancer interactions with proteins like ERBB4 further influence tumorigenic progression while in neurodegeneration WWOX interaction with Tau protein highlights its role in neuron integrity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.

See full target information WWOX

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed38203337

2024

Mechanistic Investigation of WWOX Function in NF-kB-Induced Skin Inflammation in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Jeong Shin,Hyun-Sun Kim,Pyeongan Lee,Na-Gyeong Yang,Jae-Yun Kim,Yun-Su Eun,Whiin Lee,Doyeon Kim,Young Lee,Kyung-Eun Jung,Dongkyun Hong,Jung-Min Shin,Sul-Hee Lee,Sung-Yul Lee,Chang-Deok Kim,Jung-Eun Kim

Cancer management and research 12:4379-4390 PubMed32606933

2020

LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Jun Chen,Fei Gao,Tian Yang,Hong Li,Yang Li,Hui Ren,Ming-Wei Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WWOX antibody [EPR23109-138]

Flow Cytometry (Intracellular) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (AB238144)

Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (AB238144)

Western blot - Anti-WWOX antibody [EPR23109-138] (AB238144)

Western blot - Anti-WWOX antibody [EPR23109-138] (AB238144)

Western blot - Anti-WWOX antibody [EPR23109-138] (AB238144)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise