AB231316

Anti-Xanthine Oxidase antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Xanthine Oxidase antibody. Suitable for WB and reacts with Mouse, Cow samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Cow XDH aa 1-250.

대체 명칭 보기

Xanthine dehydrogenase/oxidase, Xdh

3 이미지

Supplier Data

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

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Western blot - Anti-Xanthine Oxidase antibody (ab231316) at 1 µg/mL

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Mouse liver lysate

Secondary

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HRP-Linked Guinea pig Anti-Rabbit antibody at 1/1000 dilution

Predicted band size: 146 kDa

false

Supplier Data

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

All lanes:

Western blot - Anti-Xanthine Oxidase antibody (ab231316) at 2 µg/mL

All lanes:

Cow liver lysate

Predicted band size: 146 kDa

false

Supplier Data

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

All lanes:

Western blot - Anti-Xanthine Oxidase antibody (ab231316) at 2 µg/mL

All lanes:

Recombinant cow Xanthine Oxidase protein

Predicted band size: 146 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Cow

Applications

applications

Immunogen

Recombinant Fragment Protein within Cow XDH aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P80457

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 mg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 mg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab231316 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.

Biological function summary

Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.

Pathways

The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.

Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.

See full target information Xdh

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

European journal of nutrition 62:1845-1857 PubMed36853380

2023

Long-term dietary nitrate supplementation slows the progression of established atherosclerosis in ApoE mice fed a high fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Kevin D Croft,Trevor A Mori,Tracey A Gaspari,Barbara K Kemp-Harper,Natalie C Ward

Cell metabolism 34:1548-1560.e6 PubMed36041455

2022

PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Youn Kim,Lily Q Wang,Valentina C Sladky,Tae Gyu Oh,Junlai Liu,Kaitlyn Trinh,Felix Eichin,Michael Downes,Mojgan Hosseini,Etienne D Jacotot,Ronald M Evans,Andreas Villunger,Michael Karin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Xanthine Oxidase antibody

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise