AB123929

Anti-XPNPEP1 antibody [OTI1E3]

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(3 제품이 사용된 논문 )

Mouse Monoclonal XPNPEP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Dog, Rat, African green monkey, Transfected cell lysate - Human, Recombinant fragment - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human XPNPEP1.

대체 명칭 보기

XPNPEPL, XPNPEPL1, XPNPEP1, Xaa-Pro aminopeptidase 1, Aminoacylproline aminopeptidase, Cytosolic aminopeptidase P, Soluble aminopeptidase P, X-Pro aminopeptidase 1, sAmp

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

Paraffin-embedded human thyroid tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

Paraffin-embedded human prostate tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

All lanes:

Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

SVT2 at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 7:

MDCK at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 69 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY XPNPEP1, stained for XPNPEP1 (Green) using ab123929 at 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

All lanes:

Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA at 5 µg

Predicted band size: 69 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1E3

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human XPNPEP1.

Q9NQW7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

ab123929 is purified from Mouse ascites fluid by affinity chromatography.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The XPNPEP1 protein also known as X-prolyl aminopeptidase 1 or aminopeptidase P functions as a cytosolic enzyme and possesses a zinc-binding motif. It cleaves N-terminal amino acids from peptides containing proline. This enzyme has a mass of approximately 65 kDa. XPNPEP1 is expressed in various tissues with high expression levels observed in the kidney and liver. It operates as a monomeric enzyme and requires metal ions for its catalytic activity.

Biological function summary

XPNPEP1 plays a role in protein and peptide metabolism by processing proline-containing substrates. It does not associate with any catalytic complexes but functions autonomously to modulate peptide availability and activity. Its enzymatic activity aids in regulating peptides involved in cellular signaling and function. XPNPEP1 helps maintain homeostasis by ensuring the correct processing and degradation of proline-containing peptides which can influence numerous physiological processes.

Pathways

XPNPEP1 participates in the renin-angiotensin system (RAS) and the bradykinin degradation pathway. In the RAS XPNPEP1 modulates angiotensin molecules therefore impacting blood pressure regulation. It interacts with angiotensin-converting enzyme (ACE) within this system. In the bradykinin degradation pathway XPNPEP1 helps break down bradykinin peptides therefore preventing prolonged inflammatory responses and vascular permeability changes. Its activity is important in maintaining balance within these systems.

XPNPEP1 has links to hypertension and renal diseases due to its involvement in blood pressure and fluid balance regulation. Its association with ACE makes it relevant to conditions like hypertension where ACE inhibitors are often treatment choices. Additionally changes in XPNPEP1 expression or activity can contribute to renal dysfunction given its significant expression in kidney tissues. Research on XPNPEP1 could lead to better understanding and treatment of these cardiovascular and renal conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Metalloaminopeptidase that catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (PubMed : 11106490, PubMed : 18515364, PubMed : 35165443). Contributes to the degradation of bradykinin (PubMed : 11106490).

See full target information XPNPEP1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of medicinal chemistry 66:2589-2607 PubMed36724486

2023

Optimized M24B Aminopeptidase Inhibitors for CARD8 Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qifeng Chen,Alvin Wang,Dominic J Covelli,Abir Bhattacharjee,Qinghui Wang,Elizabeth L Orth-He,Sahana D Rao,Hsin-Che Huang,Daniel P Ball,Jeffrey C Hsiao,Daniel A Bachovchin

Nature chemical biology 18:565-574 PubMed35165443

2022

M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Sahana D Rao,Qifeng Chen,Qinghui Wang,Elizabeth L Orth-He,Michelle Saoi,Andrew R Griswold,Abir Bhattacharjee,Daniel P Ball,Hsin-Che Huang,Ashley J Chui,Dominic J Covelli,Shaochen You,Justin R Cross,Daniel A Bachovchin

Oncotarget 8:100066-100078 PubMed29245961

2017

Proteome profiling of clear cell renal cell carcinoma in von Hippel-Lindau patients highlights upregulation of Xaa-Pro aminopeptidase-1, an anti-proliferative and anti-migratory exoprotease.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Drendel,Bianca Heckelmann,Chia-Yi Chen,Juliane Weisser,Guadalupe Espadas,Christoph Schell,Eduard Sabido,Martin Werner,Cordula A Jilg,Oliver Schilling

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XPNPEP1 antibody [OTI1E3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

Western blot - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

Western blot - Anti-XPNPEP1 antibody [OTI1E3] (AB123929)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise