JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235196

Anti-XRCC1 antibody

Be the first to review this product! 리뷰 제출

|

(2 제품이 사용된 논문 )

Chicken Polyclonal XRCC1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

대체 명칭 보기

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody (AB235196)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody (AB235196)

100% methanol-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling XRCC1 using ab235196 at 10 μg/ml, overnight at +4°C. Followed by a Donkey anti-Chicken IgG DyLight™ 488 conjugated preadsorbed secondary antibody at 5 μg/ml, for 1 hour at RT.

Western blot - Anti-XRCC1 antibody (AB235196)
  • WB

Supplier Data

Western blot - Anti-XRCC1 antibody (AB235196)

All lanes:

Western blot - Anti-XRCC1 antibody (ab235196) at 5 µg/mL

Lane 1:

U-2 OS whole cell lysate at 35 µg

Lane 2:

Molt4 whole cell lysate at 35 µg

Lane 3:

Human testis whole tissue lysate at 35 µg

Lane 4:

Mouse liver whole tissue lysate at 35 µg

Secondary

All lanes:

Goat anti-Chicken IgG [H&L] at 1/40000 dilution

Predicted band size: 69 kDa

false

Exposure time: 20s

주요 정보

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB9147

Anti-XRCC1 antibody

Western blot - Anti-XRCC1 antibody (AB9147)

  • 4
  • 2
Primary Antibodies

AB134056

Anti-XRCC1 antibody [EPR4389(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

  • 5
  • 3
Primary Antibodies

AB226150

Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (AB226150)

  • 0
  • 0
Primary Antibodies

AB84417

Anti-XRCC1 (phospho S518 + T519 + T523) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 (phospho S518 + T519 + T523) antibody (AB84417)

  • 0
  • 0

보완 제품

Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 18
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB32351

Anti-Caspase-3 antibody [E87]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (AB32351)

  • 4
  • 15
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

XRCC1 (X-ray repair cross-complementing protein 1) plays a role in DNA repair by acting as a scaffold protein. It weighs approximately 70 kDa and is expressed in various tissues reflecting its role in maintaining genomic stability. XRCC1 helps in the repair of single-strand breaks by coordinating the activity of several enzymes involved in the base excision repair pathway. Together with other proteins it helps ensure the integrity of DNA by fostering repair mechanisms following damage by ionizing radiation or other environmental factors.
Biological function summary

The activity of XRCC1 integrates into DNA damage response as it partners with enzymes like DNA ligase III DNA polymerase beta and poly (ADP-ribose) polymerase (PARP). This protein functions by forming complexes with these enzymes facilitating quick and accurate repair. The ability of XRCC1 to bring together repair machinery is essential for cell survival after DNA damage and plays a role in the prevention of mutagenesis and carcinogenesis as errors in repair processes could lead to genetic mutations.

Pathways

The XRCC1 protein participates in base excision repair and single-strand break repair pathways. It works closely with PARP1 and DNA ligase III to correct single-strand DNA interruptions. Through these pathways XRCC1 interacts with other repair proteins like TDP1 aiding in the resolution of complex DNA end structures. This collaboration ensures the completion of cellular repair processes maintaining genomic integrity and reducing the likelihood of accumulating harmful mutations.

Mutations in XRCC1 have links to cancer development particularly those involving increased susceptibility to tobacco-related cancers. Studies have connected XRCC1 polymorphisms to a higher risk for conditions such as breast and lung cancer. Relations between XRCC1 and disorders can also be observed with neurodegenerative conditions; for instance alterations in its function or expression patterns might amplify disease progression involving neurological decline. Moreover its interactions with proteins like PARP1 bring forth potential implications in therapeutic strategies aiming to exploit DNA repair pathways for targeted cancer treatments.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed : 11163244, PubMed : 28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed : 28002403, PubMed : 34102106, PubMed : 34811483). Recognizes and binds poly-ADP-ribose chains : specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed : 14500814, PubMed : 34102106, PubMed : 34811483).
See full target information XRCC1
style
left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

General physiology and biophysics 44:303-315 PubMed40567073

2025

Protocatechuic acid alleviates chronic obstructive pulmonary disease model by reducing inflammation and oxidative stress via the AHR/CYP1A1/CYP1A2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Pan,Xiaowei Zhang,Ruizhi Li,Sheng Zhang,Chengda Zhang,Yongjia Zhu

Frontiers in cell and developmental biology 9:719192 PubMed34497808

2021

PARP1 Inhibitor Combined With Oxaliplatin Efficiently Suppresses Oxaliplatin Resistance in Gastric Cancer-Derived Organoids via Homologous Recombination and the Base Excision Repair Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huafu Li,Chunming Wang,Linxiang Lan,Wenhui Wu,Ian Evans,E Josue Ruiz,Leping Yan,Zhijun Zhou,Joaquim M Oliveira,Rui L Reis,Zhenran Hu,Wei Chen,Axel Behrens,Yulong He,Changhua Zhang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com