AB250301

Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Y14 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

RBM8, HSPC114, MDS014, RBM8A, RNA-binding protein 8A, Binder of OVCA1-1, RNA-binding motif protein 8A, RNA-binding protein Y14, Ribonucleoprotein RBM8A, BOV-1

3 이미지

Flow Cytometry (Intracellular) - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

This data was developed using ab181038, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab181038 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Immunoprecipitation - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

This data was developed using ab181038, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) Jurkat cell lysate or (Lane 2) 1XPBS (negative control) using ab181038 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Y14 antibody [EPR13945(B)] (<a href='/ko/products/primary-antibodies/y14-antibody-epr13945b-ab181038'>ab181038</a>)

Predicted band size: 19 kDa

false

Supplier Data

Western blot - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

This data was developed using ab181038, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Y14 antibody [EPR13945(B)] (<a href='/ko/products/primary-antibodies/y14-antibody-epr13945b-ab181038'>ab181038</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 19 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13945(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab250301 is the carrier-free version of ab181038.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Y14 also known as RBM8A is a protein integral to the exon junction complex (EJC) with an approximate mass of 18 kDa. It localizes mainly in the nucleus of cells. Y14 works with other proteins such as MAGOH serving a role in post-splicing mRNA regulation. Across various tissues Y14 shows expression but is particularly notable in tissues with high levels of mRNA processing like the brain and testis.

Biological function summary

Y14 functions in mRNA surveillance and quality control mechanisms. It forms part of the exon junction complex a multi-protein structure deposited on mRNA after splicing occurs. The component acts importantly in nonsense-mediated mRNA decay (NMD) a process that ensures defective mRNAs do not translate into dysfunctional proteins. Y14 contributes significantly to mRNA export from the nucleus binding to spliced mRNA and guiding them towards translation sites in the cytoplasm.

Pathways

Y14 plays a significant role in the nonsense-mediated mRNA decay pathway working in tandem with proteins such as UPF1 and UPF3. This pathway helps identify and degrade mRNA transcripts with premature stop codons therefore protecting cells from expressing truncated proteins that may disrupt normal cellular functions. Moreover Y14 is linked to the mRNA export pathway ensuring that only properly spliced messages reach the ribosome for translation.

Y14 relevance appears in relation to neurodevelopmental disorders and certain cancers. Mutations or dysregulation of Y14 might contribute to conditions such as intellectual disability due to its involvement in mRNA surveillance that affects neuronal development. Additionally aberrant expression of Y14 has connections to breast cancer where it interacts with proteins involved in altered signaling pathways impacting cancer progression and metastasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 28502770, PubMed : 29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

See full target information RBM8A

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

Immunoprecipitation - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

Western blot - Anti-Y14 antibody [EPR13945(B)] - BSA and Azide free (AB250301)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise