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AB114862

Anti-YAP1 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal YAP1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human YAP1 aa 400 to C-terminus.

대체 명칭 보기

YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody (AB114862)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody (AB114862)

ab114862, at 1/500 dilution, staining YAP1 in in Formalin-fixed, Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemsitry.
Detection : DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody (AB114862)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody (AB114862)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling YAP1 with ab114862at 1/100 dilution. Detection antibody was red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1 : 100. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human YAP1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46937

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대체품

Primary Antibodies

AB56701

Anti-YAP1 antibody [2F12]

Western blot - Anti-YAP1 antibody [2F12] (AB56701)

  • 5
  • 5
Primary Antibodies

AB76252

Anti-YAP1 (phospho S127) antibody [EP1675Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)

  • 4
  • 3
Primary Antibodies

AB52771

Anti-YAP1 antibody [EP1674Y]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-YAP1 antibody [EP1674Y] (AB52771)

  • 4
  • 16
Primary Antibodies

AB172373

Anti-YAP1 antibody [EP1674Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody [EP1674Y] - BSA and Azide free (AB172373)

  • 0
  • 0
Primary Antibodies

AB172374

Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (AB172374)

  • 0
  • 0
Primary Antibodies

AB62751

Anti-YAP1 (phospho Y357) antibody

Western blot - Anti-YAP1 (phospho Y357) antibody (AB62751)

  • 4
  • 1
Primary Antibodies

AB195857

HRP Anti-YAP1 antibody [EP1674Y]

RabMAb|Recombinant

Western blot - HRP Anti-YAP1 antibody [EP1674Y] (AB195857)

  • 0
  • 0

보완 제품

Assay Kits

AB230942

Human YAP1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human YAP1 ELISA Kit (AB230942)

  • 0
  • 0
Primary Antibodies

AB76252

Anti-YAP1 (phospho S127) antibody [EP1675Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] (AB76252)

  • 4
  • 3
Primary Antibodies

AB111054

Anti-LATS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody (AB111054)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB70561

Anti-LATS1/WARTS antibody

Immunoprecipitation - Anti-LATS1/WARTS antibody (AB70561)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab114862 was affinity purified using an epitope specific to YAP1 immobilized on solid support.
보관 버퍼
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.
Biological function summary

YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.

Pathways

YAP1 is importantly involved in the Hippo signaling pathway and Wnt/β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/β-catenin pathway YAP1 interacts with β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.

YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The protein expressed by the YAP1 gene functions as a transcriptional regulator, acting both as a coactivator and corepressor. It is a critical downstream regulatory target in the Hippo signaling pathway, which is crucial for organ size control and tumor suppression, as it restricts proliferation and promotes apoptosis. The pathway involves a kinase cascade where STK3/MST2 and STK4/MST1 activate LATS1/2, which then inactivates the YAP1 oncoprotein. YAP1 plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation through ARHGAP18. It controls cell proliferation in response to cell contact, with its phosphorylation by LATS1/2 preventing nuclear translocation necessary for regulating cellular genes linked to proliferation, death, and migration. TEAD transcription factors are needed for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and EMT induction. Additionally, YAP1 suppresses ciliogenesis by repressing TEAD4 target genes AURKA and PLK1. Isoforms 2 and 3 of YAP1 activate the C-terminal fragment of ERBB4 (isoform 3). This supplementary information is collated from multiple sources and compiled automatically.
See full target information YAP1
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 29:e70674 PubMed40576214

2025

Hippo Pathway Regulates Cell Proliferation in Skin Epidermis Exposed to Mechanical Forces.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna K Ledwon,Bianka Progri,Sarah A Applebaum,Oveyaa Vignesh,Alice Yau,Angie H Aguilar,Adrian B Tepole,Arun K Gosain

Heliyon 10:e27494 PubMed38515687

2024

Regulation of Dihydroartemisinin on the pathological progression of laryngeal carcinoma through the periostin/YAP/IL-6 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Zhang,Rui-Cong Li,Cong Xu,Xiao-Ming Li

Biomedicines 11: PubMed37509515

2023

Searching for Novel Biomarkers in Thymic Epithelial Tumors: Immunohistochemical Evaluation of Hippo Pathway Components in a Cohort of Thymic Epithelial Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Kostas Palamaris,Georgia Levidou,Katerina Kordali,Christos Masaoutis,Dimitra Rontogianni,Stamatios Theocharis

Clinical cancer research : an official journal of 15:2739-46 PubMed19351771

2009

CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martha S Hayden-Ledbetter,Chuck G Cerveny,Erik Espling,William A Brady,Laura S Grosmaire,Philip Tan,Robert Bader,Sonya Slater,Christy A Nilsson,Dauphine S Barone,Alexander Simon,Cheryl Bradley,Peter A Thompson,Alan F Wahl,Jeffrey A Ledbetter
제품이 사용된 논문 모두 보기
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Product promise

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