AB241612

Anti-ZC3H11A antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ZC3H11A antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZC3H11A aa 700-750.

대체 명칭 보기

KIAA0663, ZC3HDC11A, ZC3H11A, Zinc finger CCCH domain-containing protein 11A

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H11A antibody (AB241612)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H11A antibody (AB241612)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ZC3H11A using ab241612 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Immunoprecipitation - Anti-ZC3H11A antibody (AB241612)

ZC3H11A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241612 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241612 at 0.1 μg/ml.

Lane 1 : ab241612 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-ZC3H11A antibody (ab241612)

Predicted band size: 89 kDa

false

Supplier Data

Western blot - Anti-ZC3H11A antibody (AB241612)

All lanes:

Western blot - Anti-ZC3H11A antibody (ab241612) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 5 µg

Predicted band size: 89 kDa

true

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human ZC3H11A aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

O75152

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to ZC3H11A immobilized on solid support.

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ZC3H11A also known as zinc finger CCCH-type containing 11A is a protein encoded by the ZC3H11A gene. With a molecular mass of approximately 97 kDa this protein can be found expressed in various tissues with elevated levels in the brain heart and liver. Mechanically ZC3H11A functions as an RNA-binding protein participating in interactions with mRNA molecules. It contains a zinc finger domain that facilitates its interactions with nucleic acids influencing RNA stability and processing.

Biological function summary

This protein has a role in regulating mRNA stability and nuclear export. It often acts as a part of the larger mRNA export complex which includes other proteins that facilitate the transport of mRNA from the nucleus to the cytoplasm. By participating in the control of gene expression at the post-transcriptional level ZC3H11A contributes to cellular homeostasis and response to stress stimuli.

Pathways

This protein functions in the mRNA processing and transport pathways. It interacts with TREX (Transcription/Export) complex components such as Aly/REF export factor (ALYREF) to ensure proper mRNA export from the nucleus. ZC3H11A also integrates into the post-transcriptional gene regulation pathway aligning with proteins like THOC1 and THOC5 ensuring the precise control of gene expression by managing RNA fate within the cell.

Mutations or dysregulation of the ZC3H11A protein have implications in neurodegenerative diseases particularly those affecting mRNA processing and transport. For instance aberrant function of this protein has been studied in the context of Alzheimer's disease where RNA transport misregulation plays a role. ZC3H11A's connection to the THOC complex which is important in RNA metabolism links it to oncology where altered mRNA export mechanisms can contribute to cancer progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Through its association with TREX complex components, may participate in the export and post-transcriptional coordination of selected mRNA transcripts, including those required to maintain the metabolic processes in embryonic cells (PubMed : 22928037, PubMed : 37356722). Binds RNA (PubMed : 29610341, PubMed : 37356722).. (Microbial infection) Plays a role in efficient growth of several nuclear-replicating viruses such as HIV-1, influenza virus or herpes simplex virus 1/HHV-1. Required for efficient viral mRNA export (PubMed : 29610341). May be required for proper polyadenylation of adenovirus type 5/HAdV-5 capsid mRNA (PubMed : 37356722).

See full target information ZC3H11A

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:7872 PubMed39251607

2024

Systematic identification of post-transcriptional regulatory modules.

Applications

Unspecified application

Species

Unspecified reactive species

Matvei Khoroshkin,Andrey Buyan,Martin Dodel,Albertas Navickas,Johnny Yu,Fathima Trejo,Anthony Doty,Rithvik Baratam,Shaopu Zhou,Sean B Lee,Tanvi Joshi,Kristle Garcia,Benedict Choi,Sohit Miglani,Vishvak Subramanyam,Hailey Modi,Christopher Carpenter,Daniel Markett,M Ryan Corces,Faraz K Mardakheh,Ivan V Kulakovskiy,Hani Goodarzi

Journal of oncology 2020:3181596 PubMed32565799

2020

HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fang,Xu Han,Jianang Li,Tiantao Kuang,Wenhui Lou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZC3H11A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H11A antibody (AB241612)

Immunoprecipitation - Anti-ZC3H11A antibody (AB241612)

Western blot - Anti-ZC3H11A antibody (AB241612)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise