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AB226307

Anti-ZCCHC6 antibody

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Rabbit Polyclonal ZCCHC6 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TUT7 aa 1400 to C-terminus.

대체 명칭 보기

HS2, KIAA1711, ZCCHC6, TUT7, Terminal uridylyltransferase 7, TUTase 7, Zinc finger CCHC domain-containing protein 6

1 이미지
Immunoprecipitation - Anti-ZCCHC6 antibody (AB226307)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZCCHC6 antibody (AB226307)

ZCCHC6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226307 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226307 at 1 μg/ml.

Lane 1 : ab226307 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ZCCHC6 antibody (ab226307)

Predicted band size: 171 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human TUT7 aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5VYS8

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대체품

Primary Antibodies

AB76901

Anti-ZCCHC6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (AB76901)

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Primary Antibodies

AB226294

Anti-ZCCHC6 antibody

Immunoprecipitation - Anti-ZCCHC6 antibody (AB226294)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Giant Panda": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab226307 was affinity purified using an epitope specific to ZCCHC6 immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ZCCHC6 also known as Zinc finger CCHC-type containing 6 is a protein that binds RNA and has a mass of about 66 kDa. It belongs to the terminal uridylyl transferase (TUTases) family and functions by adding uridines to the 3' end of RNA substrates. This protein shows expression in multiple tissues including the brain testes and ovaries reflecting its potential involvement in diverse cellular processes.
Biological function summary

ZCCHC6 contributes to RNA metabolism influencing RNA stability translation and degradation. It participates in the post-transcriptional regulation of gene expression by adding uridines to mRNA and non-coding RNAs which can impact the decay and processing of these RNA molecules. ZCCHC6 is also part of a dynamic complex that includes components like poly(A) polymerases interacting with various cofactors to modulate RNA length and stability.

Pathways

The processes involving ZCCHC6 play significant roles in RNA processing pathways and in the regulation of gene expression. Its activity contributes to the RNA surveillance pathway which ensures the quality control of RNA transcripts. ZCCHC6 interacts with other key proteins such as ZCCHC11 which shares similar protein domains and functions in RNA uridylation reinforcing its cooperation in these pathways.

ZCCHC6 influences conditions linked to RNA dysregulation such as certain cancers and neurodegenerative diseases. The protein plays a role in maintaining RNA quality and abundance and disturbances in its function can lead to abnormal RNA profiles observed in these disorders. In cancer for example aberrant activity of ZCCHC6 might disrupt normal cell regulatory mechanisms potentially interacting with proteins like LIN28 to promote oncogenic processes.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed : 19703396, PubMed : 25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed : 25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed : 25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed : 22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed : 25979828, PubMed : 28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed : 25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed : 18172165, PubMed : 19703396, PubMed : 22898984, PubMed : 25480299, PubMed : 25979828, PubMed : 28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed : 30122351).
See full target information TUT7
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in aging neuroscience 14:926904 PubMed35978952

2022

Intraneuronal sortilin aggregation relative to granulovacuolar degeneration, tau pathogenesis and sorfra plaque formation in human hippocampal formation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Jiang,Chen Yang,Jia-Qi Ai,Qi-Lei Zhang,Xiao-Lu Cai,Tian Tu,Lily Wan,Xiao-Sheng Wang,Hui Wang,Aihua Pan,Jim Manavis,Wei-Ping Gai,Chong Che,Ewen Tu,Xiao-Ping Wang,Zhen-Yan Li,Xiao-Xin Yan
제품이 사용된 논문 모두 보기
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Product promise

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