AB235369

Anti-ZDHHC4/DC1 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal ZDHHC4/DC1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC4 aa 250 to C-terminus.

대체 명칭 보기

ZNF374, DC1, UNQ5787/PRO19576, ZDHHC4, Palmitoyltransferase ZDHHC4, Zinc finger DHHC domain-containing protein 4, Zinc finger protein 374, DHHC-4

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas tissue labelling ZDHHC4/DC1 with ab235369 at a 1 : 50 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab235369 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

Supplier Data

Western blot - Anti-ZDHHC4/DC1 antibody (AB235369)

All lanes:

Western blot - Anti-ZDHHC4/DC1 antibody (ab235369) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

SY5Y whole cell lysate at 30 µg

Lane 4:

MCF7 whole cell lysate at 30 µg

Lane 5:

HT29 whole cell lysate at 30 µg

Lane 6:

Mouse Liver tissue lysate at 30 µg

Lane 7:

Rat Lung tissue lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Observed band size: 40 kDa

false

Exposure time: 120s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZDHHC4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NPG8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ZDHHC4 also known as DC1 is a palmitoyltransferase enzyme with a mass of approximately 35 kDa. It plays an important role in attaching palmitate a fatty acid to cysteine residues on target proteins which is an important post-translational modification. This modification helps in the trafficking stability and function of proteins within a cell. ZDHHC4 is expressed in various tissues including the brain and liver influencing diverse biological processes and cellular functions.

Biological function summary

This palmitoyltransferase influences protein-protein interactions by adding lipid moieties to key signaling molecules. It is part of the ZDHHC family of proteins which collaborate to modify a variety of substrates important for cellular signaling pathways. By palmitoylating proteins ZDHHC4 can regulate pathways involving signal transduction and vesicular trafficking affecting how signals are transmitted inside the cell and how proteins are transported to different cellular compartments.

Pathways

Research shows this enzyme acts within the signaling pathways such as the Wnt signaling and Ras signaling. In the Wnt pathway ZDHHC4's palmitoylation activity helps control the stability and localization of proteins important for signal propagation. Within the Ras signaling pathway it modifies Ras proteins which are related to cell growth and survival. These associations highlight its role in influencing cell behavior through complex signaling networks.

Mutations or dysregulation of ZDHHC4 have links to neurodegenerative diseases and cancer. For instance its activity impacts protein interactions that when impaired can contribute to Alzheimer's disease. In cancer ZDHHC4's altered palmitoylation activity can affect oncogenic signaling cascades contributing to tumor progression. It shows interaction with proteins such as beta-catenin in cancer and Beta-amyloid precursor in Alzheimer's connecting ZDHHC4 to these diseases at a molecular level.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Palmitoyltransferase that catalyzes the addition of palmitate onto protein substrates including the D(2) dopamine receptor DRD2, GSK3B or MAVS. Mediates GSK3B palmitoylation to prevent its AKT1-mediated phosphorylation leading to activation of the STAT3 signaling pathway (PubMed : 35606353). Also catalyzes MAVS palmitoylation which promotes its stabilization and activation by inhibiting 'Lys-48'- but facilitating 'Lys-63'-linked ubiquitination (PubMed : 38016475).

See full target information ZDHHC4

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 10:eado3141 PubMed39047111

2024

Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwei Hu,Rui Li,Dongxu Gong,Pei Hu,Jitu Xu,Yingjie Ai,Xiaojie Zhao,Chencheng Hu,Minghuan Xu,Chenxi Liu,Shuyu Chen,Jie Fan,Zhonghua Zhao,Zhigang Zhang,Huijuan Wu,Yanyong Xu

PLoS pathogens 20:e1012379 PubMed39037956

2024

The RNA helicase DHX35 functions as a co-sensor for RIG-I-mediated innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Qiao,Shan Zhu,Ning Yang,Shan-Shan Zou,Bao Gao,Jing Wu,Chunyan Liu,Xiaoping Li,Yong-Jun Liu,Jingtao Chen

Oncogenesis 11:28 PubMed35606353

2022

GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenggang Zhao,Huihan Yu,Xiaoqing Fan,Wanxiang Niu,Junqi Fan,Suling Sun,Meiting Gong,Bing Zhao,Zhiyou Fang,Xueran Chen

Journal of inherited metabolic disease 45:635-656 PubMed35150145

2022

Ppt1-deficiency dysregulates lysosomal Ca homeostasis contributing to pathogenesis in a mouse model of CLN1 disease.

Applications

Unspecified application

Species

Unspecified reactive species

Avisek Mondal,Abhilash P Appu,Tamal Sadhukhan,Maria B Bagh,Rafael M Previde,Sriparna Sadhukhan,Stanko Stojilkovic,Aiyi Liu,Anil B Mukherjee

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZDHHC4/DC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

Western blot - Anti-ZDHHC4/DC1 antibody (AB235369)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise