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AB74504

Anti-ZDHHC9 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal ZDHHC9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZDHHC9.
2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC9 antibody (AB74504)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC9 antibody (AB74504)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74504. Left : untreated sample. Right : sample treated with immunising peptide.

Western blot - Anti-ZDHHC9 antibody (AB74504)
  • WB

Unknown

Western blot - Anti-ZDHHC9 antibody (AB74504)

All lanes:

Western blot - Anti-ZDHHC9 antibody (ab74504) at 1/500 dilution

Lane 1:

extracts from COLO cells at 30 µg

Lane 2:

extracts from COLO cells at 30 µg with immunising peptide

Predicted band size: 41 kDa

Observed band size: 26 kDa,34 kDa,41 kDa,48 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ZDHHC9. The exact immunogen used to generate this antibody is proprietary information.

Q9Y397

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대체품

Primary Antibodies

AB217757

Anti-ZDHHC9 antibody

  • 0
  • 0

보완 제품

Primary Antibodies

AB138210

Anti-ZDHHC7 antibody

  • 1
  • 1
Primary Antibodies

AB76020

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB76020)

  • 5
  • 18
Primary Antibodies

AB176842

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade

20ul selling size|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (AB176842)

  • 5
  • 15
Primary Antibodies

AB28759

Anti-ZDHHC13 antibody

Western blot - Anti-ZDHHC13 antibody (AB28759)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ZDHHC9 also known as DHHC9 is a protein that functions as a palmitoyltransferase catalyzing the addition of palmitate a fatty acid to cysteine residues on substrate proteins. This post-translational modification known as S-palmitoylation regulates protein stability localization and interactions. ZDHHC9 has a molecular weight of approximately 39 kDa and is widely expressed in various tissues including the brain and heart. The protein localizes to the Golgi apparatus and affects the membrane association of its substrate proteins.
Biological function summary

ZDHHC9 influences cellular signaling and protein trafficking. It forms a complex with GCP16 another subunit enhancing its palmitoylation activity. ZDHHC9 regulates the palmitoylation of proteins involved in signaling pathways such as Ras proteins. This modification affects Ras proteins' membrane localization and function impacting cellular communication and signaling events. By modulating these pathways ZDHHC9 plays a part in maintaining normal cell physiology.

Pathways

ZDHHC9 modifies signal transduction pathways like the MAPK/ERK and the NF-κB pathways. The palmitoylated proteins especially H-Ras and N-Ras are integral to these signaling pathways that control cell growth differentiation and survival. The enzyme’s activity affects the dynamic regulation of these pathways influencing how cells respond to external stimuli. By interacting with these Ras proteins ZDHHC9 indirectly impacts pathways that regulate essential cellular processes.

Mutations or dysregulation of ZDHHC9 have associations with mental retardation and X-linked cognitive impairment. These conditions relate to altered Ras signaling where ZDHHC9 mutations impact normal Ras palmitoylation and function. Moreover defects in this protein have connections to developmental abnormalities due to disruptions in signal transduction during brain development. Understanding ZDHHC9's interactions with proteins like Ras provides insight into these complex disorders and potential therapeutic targets.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as ADRB2, GSDMD, HRAS, NRAS and CGAS (PubMed : 16000296, PubMed : 27481942, PubMed : 37802025, PubMed : 38530158, PubMed : 38599239). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS (PubMed : 16000296). May have a palmitoyltransferase activity toward the beta-2 adrenergic receptor/ADRB2 and therefore regulate G protein-coupled receptor signaling (PubMed : 27481942). Acts as a regulator of innate immunity by catalyzing palmitoylation of CGAS, thereby promoting CGAS homodimerization and cyclic GMP-AMP synthase activity (PubMed : 37802025). Activates pyroptosis by catalyzing palmitoylation of gasdermin-D (GSDMD), thereby promoting membrane translocation and pore formation of GSDMD (PubMed : 38530158, PubMed : 38599239).. (Microbial infection) Through a sequential action with ZDHHC20, rapidly and efficiently palmitoylates SARS coronavirus-2/SARS-CoV-2 spike protein following its synthesis in the endoplasmic reticulum (ER). In the infected cell, promotes spike biogenesis by protecting it from premature ER degradation, increases half-life and controls the lipid organization of its immediate membrane environment. Once the virus has formed, spike palmitoylation controls fusion with the target cell.
See full target information ZDHHC9

대체 명칭 보기

CXorf11, ZDHHC10, ZNF379, ZNF380, CGI-89, UNQ261/PRO298, ZDHHC9, Palmitoyltransferase ZDHHC9, Zinc finger DHHC domain-containing protein 9, Zinc finger protein 379, Zinc finger protein 380, DHHC-9, DHHC9
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 25:5 PubMed36419754

2022

ZDHHC9 promotes colon tumor growth by inhibiting effector T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Chong,Lingxi Zhu,Dong Yu,Shasha Chen,Guang Wang,Qingzhuo Yu,Xianwei Ma,Jing Xu,Haitao Chen,Huazhang An

RSC chemical biology 2:192-205 PubMed34458782

2021

A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions.

Applications

Unspecified application

Species

Unspecified reactive species

Han Yao,Chushu Li,Fang He,Teng Song,Jean-Philippe Brosseau,Huanbin Wang,Haojie Lu,Caiyun Fang,Hubing Shi,Jiang Lan,Jing-Yuan Fang,Jie Xu

Proceedings of the National Academy of Sciences of the United States of America 109:20473-8 PubMed23184980

2012

Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach.

Applications

WB

Species

Unspecified reactive species

Xiaolu A Cambronne,Rongkun Shen,Paul L Auer,Richard H Goodman
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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