AB327146

Anti-ZEB2 Antibody [CL13467]

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Mouse Monoclonal ZEB2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB2.

대체 명칭 보기

KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1

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Immunocytochemistry/ Immunofluorescence - Anti-ZEB2 Antibody [CL13467] (AB327146)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunofluorescence staining in U-87 cell line using ab327146 at 2–10 µg/ml, showing specific staining of nuclear speckles in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human tonsil using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in germinal center cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human cervix using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human cerebral cortex using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327146 at 1 : 20–1 : 50 dilution. Corresponding ZEB2 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human anaplastic oligodendroglioma using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in tumor cells.

Supplier Data

Western blot - Anti-ZEB2 Antibody [CL13467] (AB327146)

Western blot analysis in human cell lines SK-MEL-30 and Caco-2 using ab327146 at 1 µg/ml. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. Loading control : Anti-HDAC1 antibody.

All lanes:

Western blot - Anti-ZEB2 Antibody [CL13467] (ab327146)

Lane 2:

Human cell line SK-MEL-30

Lane 3:

Human cell line Caco-2

Predicted band size: 136.45 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human skeletal muscle using ab327146 at 1 : 20–1 : 50 dilution, showing no positivity in myocytes as expected.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL13467

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZEB2.

O60315

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed : 16061479, PubMed : 20516212). Represses transcription of E-cadherin (PubMed : 16061479). Represses expression of MEOX2 (PubMed : 20516212).

See full target information ZEB2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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