AB96083

Anti-ZHX2 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ZHX2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZHX2 aa 500-800.

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96083 at 1/200 dilution. (1) ab96083, (2) Merged with DNA probe

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ZHX2 protein at nucleus using ab96083 at a 1/500 dilution (Green). Phalloidin, a cytoskeleton marker, was stained at a 1/200 dilution (Red).
Scale bar = 10 µM

Supplier Data

Immunoprecipitation - Anti-ZHX2 antibody (AB96083)

Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 ug of ab96083.
Western blot analysis was performed using ab96083.
Anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-ZHX2 antibody (ab96083) at 5 µg

Lane 1:

MCF-7 whole cell extracts

Lane 2:

MCF-7 whole cell extracts with Control IgG

Lane 3:

MCF-7 whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG antibody

Predicted band size: 92 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZHX2 aa 500-800. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6X8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Zinc fingers and homeoboxes protein 2 (ZHX2) is a transcriptional repressor with an approximate molecular mass of 95 kDa. ZHX2 is expressed in various tissues including the liver kidney and spleen and it plays a role in regulating gene expression by binding to specific DNA sequences. Also known as zinc fingers and homeoboxes protein 2 ZHX2 contains two C2H2-type zinc finger domains and four homeobox domains which enable its DNA-binding ability and interaction with other proteins.

Biological function summary

ZHX2 impacts gene regulation by interacting with other transcription factors. It is known to associate with ZHX1 and NF-YA to form a protein complex that modulates the expression of various target genes. Through these interactions ZHX2 influences cell division differentiation and survival. It can act as a transcriptional repressor by binding to promoter regions of genes therefore modulating their expression levels in cellular contexts.

Pathways

ZHX2 influences several biological processes. It is involved in the Wnt signaling pathway where it can influence cellular proliferation and differentiation by regulating the expression of target genes. Additionally ZHX2 interacts with the pathways governed by the transforming growth factor-beta (TGF-β) family which are important for cellular proliferation differentiation and apoptosis. ZHX2 can modulate these pathways by interacting with proteins such as SMAD2/3 and β-catenin impacting their downstream effects.

ZHX2 has been linked to cancer progression and autoimmune diseases. In certain cancers ZHX2 expression levels correlate with tumor progression suggesting it may serve as a potential marker or therapeutic target. ZHX2 influences the expression of oncogenes and tumor suppressor genes in this context. In autoimmune conditions it has been implicated in abnormal immune responses potentially by regulating genes involved in immune cell function. Through these diseases ZHX2 is associated with proteins like p53 in cancer and NF-κB in autoimmune disorders affecting their signaling and regulatory roles.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a transcriptional repressor (PubMed : 12741956). Represses the promoter activity of the CDC25C gene stimulated by NFYA (PubMed : 12741956). May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity).

See full target information ZHX2

대체 명칭 보기

AFR1, KIAA0854, RAF, ZHX2, Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2, AFP regulator 1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Hepatology research : the official journal of the Japan Society of Hepatology : PubMed39207766

2024

Elevated nuclear expression of ZHX1 correlates with poor prognosis in hepatocellular carcinoma (HCC): Comparison of nuclear and cytoplasmic distribution of the ZHX family in HCC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hong Ma,Shinya Maekawa,Shinichi Takano,Tatsuya Yamaguchi,Takeshi Ishida,Shinya Takaoka,Masaru Muraoka,Yasuyuki Komiyama,Hitomi Takada,Yuichiro Suzuki,Mitsuaki Sato,Jianglin Fan,Nobuyuki Enomoto

Gene expression 17:7-17 PubMed27197076

2016

Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver.

Applications

IHC-Fr

Species

Mouse

Kate T Creasy,Jieyun Jiang,Hui Ren,Martha L Peterson,Brett T Spear

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZHX2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

Immunoprecipitation - Anti-ZHX2 antibody (AB96083)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise