AB250225

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Zinc Alpha 2 Glycoprotein antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (<a href='/ko/products/primary-antibodies/zinc-alpha-2-glycoprotein-antibody-epr14425b-ab180574'>ab180574</a>) at 1/30 dilution

Lane 1:

Human plasma

Lane 2:

Negative control

Secondary

All lanes:

Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (<a href='/ko/products/primary-antibodies/zinc-alpha-2-glycoprotein-antibody-epr14425b-ab180574'>ab180574</a>) at 1/20000 dilution

All lanes:

Human plasma at 20 µg

Secondary

All lanes:

Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (<a href='/ko/products/primary-antibodies/zinc-alpha-2-glycoprotein-antibody-epr14425b-ab180574'>ab180574</a>) at 1/2000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/1000 dilution

Predicted band size: 34 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14425(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab250225 is the carrier-free version of ab180574.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Zinc Alpha 2 Glycoprotein (ZAG) also called ZAG or AZGP1 is a soluble protein with a molecular mass of approximately 41 kDa. It functions as an adipokine and is mostly found in the liver breast and prostate tissues as well as in bodily fluids like plasma and saliva. ZAG belongs to the major histocompatibility complex class I family and it shares structural similarities with antigen-presenting proteins.

Biological function summary

Zinc Alpha 2 Glycoprotein assists in lipid metabolism. It promotes the breakdown of triglycerides in adipose tissue by stimulating the beta-adrenergic pathway. ZAG functions independently and does not typically form complexes with other proteins during this process. Its presence in adipose tissue suggests a role in energy homeostasis and body weight regulation.

Pathways

Zinc Alpha 2 Glycoprotein participates in the metabolic pathway for lipid degradation. The protein interacts with beta-3 adrenergic receptors to trigger lipolysis which involves breaking down fat cells into free fatty acids and glycerol. Its action in this pathway links ZAG with other proteins such as perilipin which protects lipid droplets from breakdown.

Zinc Alpha 2 Glycoprotein has connections with metabolic-related conditions notably obesity and cancer cachexia. In obesity lower levels of ZAG are often observed potentially linking it to impaired fat metabolism. In cancer cachexia elevated ZAG levels are frequently detected suggesting a role in the loss of fat and muscle tissue. ZAG's interaction with certain cytokines may enhance cachexia progression by affecting protein degradation pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.

See full target information AZGP1

대체 명칭 보기

ZAG, ZNGP1, AZGP1, Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise