AB248299

Anti-ZNF136 antibody [EPR8225] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ZNF136 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

Zinc finger protein 136, ZNF136

2 이미지

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Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

This data was developed using ab129100, the same antibody clone in a different buffer formulation.

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Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299) at 1/1000 dilution

Lane 4:

Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)

Lane 1:

Human fetal heart cell lysates at 10 µg

Lane 4:

HeLa cell lysates

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 63 kDa

false

OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

OI-RD Scanning

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OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8225

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab248299 is the carrier-free version of ab129100.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ZNF136 also known as Zinc Finger Protein 136 functions as a transcription factor due to its zinc finger domains which allow it to bind specific DNA sequences. It weighs approximately 65 kDa. This protein regulates the expression of target genes by interacting with DNA in a sequence-specific manner. Scientists find it predominantly expressed in the brain with lower levels detected in other tissues suggesting a specialized role in neural processes.

Biological function summary

ZNF136 regulates genes implicated in neuronal development and function. It may form part of transcriptional complexes but this requires further study. ZNF136 impacts the expression of genes necessary for maintaining neural network integrity and synaptic plasticity. Its activity supports proper cognitive function and neural communication indicating it plays a role in the regulation of brain homeostasis.

Pathways

ZNF136 integrates into neural gene regulation networks. It influences the Wnt signaling pathway which is important in neuronal development and function and interacts with proteins such as Beta-Catenin. Additionally ZNF136 participates in the transcriptional regulation of genes within the Notch signaling pathway working with proteins like RBPJ to fine-tune responses required for cell differentiation and maturation.

ZNF136 has associations with neurological conditions like schizophrenia and autism spectrum disorders. Abnormal expression or mutations in ZNF136 may disrupt neural communication pathways contributing to disease pathogenesis. It also seems related to dysregulation of proteins like NRXN1 which is implicated in synaptic connectivity providing insight into how ZNF136 alterations could influence these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.

See full target information ZNF136

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF136 antibody [EPR8225] - BSA and Azide free

Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise