AB204974

Anti-ZNF471/ERP1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal ZNF471/ERP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF471 aa 50 to C-terminus.

대체 명칭 보기

ERP1, KIAA1396, ZNF471, Zinc finger protein 471, EZFIT-related protein 1

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunocytochemistry/ Immunofluorescence - Anti-ZNF471/ERP1 antibody (AB204974)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF471/ERP1 antibody (AB204974)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling ZNF471/ERP1 in the nucleus with ab204974 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ZNF471/ERP1 in the cytoplasm and nucleus of glandular cells with ab204974 at a 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ZNF471/ERP1 antibody (AB204974)

All lanes:

Western blot - Anti-ZNF471/ERP1 antibody (ab204974) at 0.4 µg/mL

All lanes:

U-2 OS cells

Predicted band size: 73 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZNF471 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BX82

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ZNF471 also known as ERP1 is a zinc finger protein that plays a significant mechanical role in cellular processes. It has an approximate molecular weight of around 75 kDa. The protein is expressed in various tissues with a higher presence observed in epithelial tissues. As a zinc finger protein ZNF471 binds to DNA influencing transcriptional regulation. ZNF471/ERP1 is important in maintaining the integrity of gene expression by interacting with specific DNA sequences.

Biological function summary

This zinc finger protein regulates transcription factors and affects gene expression patterns. ZNF471/ERP1 does not exist as part of a larger protein complex allowing it to function more independently. Through its ability to bind DNA it influences the transcriptional activity of multiple key genes involved in cellular growth differentiation and development. It has a significant role in signaling pathways linked to cellular proliferation.

Pathways

ZNF471/ERP1 interacts with transcriptional machinery to regulate the Wnt/β-catenin and TP53 pathways. Within these pathways it plays a regulator role modulating gene expressions necessary for cellular growth and apoptosis. For instance it acts in concert with proteins such as β-catenin and TP53 to either promote or inhibit the transcription of genes depending on the cellular context. These regulatory activities highlight its importance in maintaining cellular homeostasis.

Aberrations in ZNF471/ERP1 expression have associations with cancer specifically colorectal and breast cancer. Dysregulation can lead to uncontrolled cellular proliferation and resistance to apoptosis which are hallmarks of cancer initiation and progression. The protein's interaction with other pivotal proteins such as TP53 and β-catenin in these cancers indicates its therapeutic and diagnostic potential in oncology as researchers explore targeted treatments that may influence this interaction pathway.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May be involved in transcriptional regulation.

See full target information ZNF471

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical epigenetics 12:173 PubMed33203470

2020

The tumor suppressor Zinc finger protein 471 suppresses breast cancer growth and metastasis through inhibiting AKT and Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfang Tao,Juan Luo,Jun Tang,Danfeng Zhou,Shujun Feng,Zhu Qiu,Thomas C Putti,Tingxiu Xiang,Qiao Tao,Lili Li,Guosheng Ren

Cancer cell international 20:180 PubMed32489316

2020

lncRNA LIFR-AS1 suppresses invasion and metastasis of non-small cell lung cancer via the miR-942-5p/ZNF471 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Wang,Jing Wu,Hui Huang,Yan Jiang,Ying Huang,Hongyan Fang,Gang Zheng,Xiaochun Zhou,Yujuan Wu,Changjiang Lei,Desheng Hu

Molecular cancer research : MCR 14:841-8 PubMed27280713

2016

Downregulation of the Deiminase PADI2 Is an Early Event in Colorectal Carcinogenesis and Indicates Poor Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Neus Cantariño,Eva Musulén,Vanesa Valero,Miquel Angel Peinado,Manuel Perucho,Victor Moreno,Sònia-Vanina Forcales,Julien Douet,Marcus Buschbeck

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF471/ERP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunocytochemistry/ Immunofluorescence - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Western blot - Anti-ZNF471/ERP1 antibody (AB204974)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise