AB250095

Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ZNFX1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

KIAA1404, ZNFX1, NFX1-type zinc finger-containing protein 1

2 이미지

Supplier Data

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)

This data was developed using ab179452, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNFX1 antibody [EPR12330] (<a href='/ko/products/primary-antibodies/znfx1-antibody-epr12330-ab179452'>ab179452</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 220 kDa

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Unknown

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)

This data was developed using ab179452, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNFX1 antibody [EPR12330] (<a href='/ko/products/primary-antibodies/znfx1-antibody-epr12330-ab179452'>ab179452</a>) at 1/1000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 220 kDa

Observed band size: 220 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12330

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab250095 is the carrier-free version of ab179452.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger NFX1-type containing 1 (ZNFX1) protein is a DNA-binding protein involved in nucleic acid sensing and immune regulation. It is also known as ZNF-X1. ZNFX1 has a mass of approximately 119 kDa. It expresses in various tissues with notably higher expression levels in the spleen lymph nodes and blood cells. The protein features zinc finger domains which facilitate its interaction with nucleic acids enabling ZNFX1 to play a role in transcriptional regulation and the antiviral response.

Biological function summary

ZNFX1 influences immune function and viral defense mechanisms. Acting within ribonucleoprotein complexes it participates in the sensing of double-stranded RNA an indicator of viral infection. ZNFX1 helps modulate type I interferon responses a critical aspect of host defense. In these processes it interacts with other proteins necessary for the immune system contributing to maintaining cellular immune surveillance and controlling viral replication.

Pathways

The actions of ZNFX1 integrate into pathways related to RNA processing and immune responses. Specifically ZNFX1 is involved in the RIG-I-like receptor (RLR) pathway which is important for antiviral responses. In this pathway ZNFX1 interacts with proteins like RIG-I and MAVS to facilitate the proper functioning of antiviral signaling cascades. This pathway's coordination leads to the activation of transcription factors that promote the expression of type I interferons and other cytokines essential for antiviral immunity.

ZNFX1 associates with immunological conditions including systemic lupus erythematosus (SLE). Abnormalities in ZNFX1 function or expression may contribute to the dysregulation of immune response observed in this autoimmune disease. Furthermore ZNFX1 connects with proteins such as TREX1 known to be involved in the pathogenesis of SLE through the management of immune signaling and prevention of excessive immune activation. Understanding these relationships highlights the significance of ZNFX1 in both normal and disease states of the immune system.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

RNA-binding protein that initiates the antiviral response and is required to restrict the replication of RNA viruses (PubMed : 33872655). Acts as a double-stranded RNA (dsRNA) sensor that recognizes viral RNA and then interacts with MAVS to initiate the type I interferon response (By similarity). Also required for immunity against some bacteria, such as mycobacteria (PubMed : 33876776).

See full target information ZNFX1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise