AB244277

Anti-Zyxin antibody

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Rabbit Polyclonal Zyxin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZYX aa 250 to C-terminus.

대체 명칭 보기

Zyxin, Zyxin-2, ZYX

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody (AB244277)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody (AB244277)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Zyxin using ab244277 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody (AB244277)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody (AB244277)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Zyxin (green) using ab244277 at 4 μg/ml in ICC/IF.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

Immunogen

Recombinant Fragment Protein within Human ZYX aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB78910

Anti-Zyxin (phospho S142) antibody

Western blot - Anti-Zyxin (phospho S142) antibody (AB78910)

0
0

Primary Antibodies

AB109316

Anti-Zyxin antibody [EPR4302]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Zyxin antibody [EPR4302] (AB109316)

0
0

Primary Antibodies

AB238430

Anti-Zyxin antibody [EPR4302] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (AB238430)

0
0

Primary Antibodies

AB50391

Anti-Zyxin antibody [ZOL301]

KO Validated

Western blot - Anti-Zyxin antibody [ZOL301] (AB50391)

5
1

Primary Antibodies

AB58210

Anti-Zyxin antibody [2D1]

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (AB58210)

4
1

보완 제품

Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

5
21

Primary Antibodies

AB150361

Anti-Integrin alpha 5 antibody [EPR7854]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (AB150361)

5
13

Primary Antibodies

AB129002

Anti-Vinculin antibody [EPR8185] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated

5
21

Primary Antibodies

AB314500

Anti-Lamin C antibody [EPR28295-16] - Nuclear Envelope Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin C antibody [EPR28295-16] - Nuclear Envelope Marker (AB314500)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Zyxin also known as ZYX is a mechanical regulator associated with cell adhesion and signaling. This protein has a molecular mass of approximately 61 kDa and shows expression in various cell types including those in the heart liver and lungs. As a component of focal adhesions zyxin plays a role in cytoskeletal dynamics by interacting with actin filaments and facilitating the formation of stress fibers.

Biological function summary

The protein has important roles in cellular processes such as migration and morphology. Zyxin often forms part of a larger protein complex that includes VASP and α-actinin which contribute to cell scaffolding and structural integrity. It serves as an organizer for cytoskeletal proteins ensuring proper cell movement and adaptability to cellular stress. Its actin-binding capacity enables it to regulate structural changes necessary for cellular adaptation to environmental stimuli.

Pathways

This protein is essential in the regulation of actin cytoskeleton associated pathways and adherens junction signaling. Zyxin interacts with Ena/VASP proteins which are significant in actin filament elongation helping regulate dynamic changes in cell shape and motility. Its relationship with LIM domain-containing proteins positions zyxin within pathways related to cell proliferation and signal transduction.

Zyxin has been implicated in cancer progression especially in metastasis where altered cell adhesion and movement play a role. It also relates to cardiovascular diseases due to its involvement in mechanotransduction pathways critical to heart muscle function. In cancer zyxin influences the activity of YAP/TAZ transcription regulators affecting tumor cell behavior. In cardiovascular scenarios the protein interacts with paxillin to mediate responses to mechanical load changes impacting heart disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

See full target information ZYX

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 43:114309 PubMed38848215

2024

Lineage specification in glioblastoma is regulated by METTL7B.

Applications

Unspecified application

Species

Unspecified reactive species

Myrianni Constantinou,James Nicholson,Xinyu Zhang,Eleni Maniati,Sara Lucchini,Gabriel Rosser,Claire Vinel,Jun Wang,Yau Mun Lim,Sebastian Brandner,Sven Nelander,Sara Badodi,Silvia Marino

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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