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AB51992

EGFP protein (His tag)

EGFP 단백질 (His tag)

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EGFP protein (His tag) is a recombinant Aequorea victoria Full Length protein, in the 2 to 238 aa range, with >=97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, western blot and conjugation assays. The predicted molecular weight of ab51992 protein is 32.7 kDa.

- ideal for use as a positive control
- Contains N-terminal and C-terminal His tags
- Available in different sizes to fit your experimental needs

See more GFP products

대체 명칭 보기

Green fluorescent protein, GFP

주요 정보

Purity

>97% SDS-PAGE

Endotoxin 레벨

< 1 EU/µg

발현 시스템

Escherichia coli

Tags

His tag C-Terminus His tag N-Terminus

Applications

SDS-PAGE, Conjugation, WB

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Aequorea victoria

Reconstitution

Reconstitute in PBS

보관 버퍼

Constituents: PBS

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

  • 0
  • 0
Proteins & Peptides

AB199750

Recombinant mCherry protein (His tag)

  • 4
  • 1
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB171581

GFP ELISA Kit

SimpleStep

Sandwich ELISA - GFP ELISA Kit (AB171581)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Conjugation": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Ensure the validity of your result using our EGFP protein (His tag) ab51992 as a control in SDS-PAGE and western blot.

This Enhanced GFP (eGFP) protein is suitable as control reagent for GFP expression studies. Other applications include: calibration of fluorometers and flow cytometers, fluorescence microscopy, and microinjection of GFP into cells and tissues, etc.

The excitation and emmission spectra for this ab51992 recombinant EGFP is Ex/Em = 488/509 nm.


Check out our protein gel staining guide for SDS-PAGE here

Check out our western blot protocol for more information here


This product is manufactured by BioVision, an Abcam company and was previously called 4999 Enhanced Green Fluorescent Protein (EGFP). 4999-100 is the same size as the 100 µg size of ab51992.
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMASVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKASMTGGQQMGRDPNSSSVDKLAAALEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"32.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

서열 유사성

Belongs to the GFP family.

Post-translational modifications

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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제품 프로토콜

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타겟 정보

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

ACS nano 19:5240-5252 PubMed39871506

2025

Large and Stable Nanopores Formed by Complement Component 9 for Characterizing Single Folded Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Wachara Chanakul,Anasua Mukhopadhyay,Saurabh Awasthi,Anna D Protopopova,Alessandro Ianiro,Michael Mayer
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com