AB91136

Native human alpha 1 Antitrypsin protein (Active)

Be the first to review this product! Submit a review

(2 Publications)

Native human alpha 1 Antitrypsin protein (Active) is a Human Full Length A1AT/SERPIN A1 protein, with >95% purity and suitable for western blot , SDS-PAGE and Functional studies. The predicted molecular weight of ab91136 native protein is 52 kDa.

- Save time and ensure accurate results - use our A1AT/SERPIN A1 protein
- Optimal protein bioactivity and stability

대체 명칭 보기

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

2 Images

Unknown

Western blot - Native human alpha 1 Antitrypsin protein (Active) (AB91136)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (<a href='/ko/products/primary-antibodies/alpha-1-antitrypsin-antibody-b9-ab9399'>ab9399</a>) at 1 µg/mL

All lanes:

Western blot - Native human alpha 1 Antitrypsin protein (Active) (ab91136) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 46 kDa

true

Exposure time: 30s

SDS-PAGE - Native human alpha 1 Antitrypsin protein (Active) (AB91136)

SDS-PAGE

Unknown

SDS-PAGE - Native human alpha 1 Antitrypsin protein (Active) (AB91136)

SDS-PAGE : 4-12% Bis-Tris NuPAGE gel

Lane 1. 5 μg ab91136 (reduced/heated)
Lane 2. 10 μg ab91136 (reduced/heated)
Lane 3. 20 μg ab91136 (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg ab91136 (non-reduced/no heat)
Lane 6. 10 μg ab91136 (non-reduced/no heat)
Lane 7. 20 μg ab91136 (non-reduced/no heat)

주요 정보

Purity

>95% SDS-PAGE

발현 시스템

Native

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inhibitory.

Accession

P01009

Animal free

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in water

보관 버퍼

pH: 6.5 Constituents: 1.74% Sodium chloride, 0.492% Sodium phosphate

storage-buffer

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab91136 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/alpha-1-antitrypsin-antibody-b9-ab9399'>ab9399</a>." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

style

left-column

제품 세부 정보

The Native human alpha 1 Antitrypsin protein (Active) ab91136 is prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Ensure the validity of your result using our native human alpha 1 Antitrypsin (A1AT/SERPIN A1) protein as a control in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here

Protein Determination: Extinction Coefficient (ε) 0.1% at 280 nm, 1 cm pathway = 0.433

style

left-column

서열 정보

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"52 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P01009","tags":[]}]

style

left-column

특성 및 보관 정보

제형

Lyophilized

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

True

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

style

left-column

일반 정보

기능

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

서열 유사성

Belongs to the serpin family.

Post-translational modifications

N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.

style

left-column

제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

See full target information SERPINA1

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature genetics 51:1233-1243 PubMed31358993

2019

CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemehsadat Esteghamat,James S Broughton,Emily Smith,Rebecca Cardone,Tarun Tyagi,Mateus Guerra,András Szabó,Nelson Ugwu,Mitra V Mani,Bani Azari,Gerald Kayingo,Sunny Chung,Mohsen Fathzadeh,Ephraim Weiss,Jeffrey Bender,Shrikant Mane,Richard P Lifton,Adebowale Adeniran,Michael H Nathanson,Fred S Gorelick,John Hwa,Miklós Sahin-Tóth,Renata Belfort-DeAguiar,Richard G Kibbey,Arya Mani

Journal of neuroinflammation 14:168 PubMed28835277

2017

Prophylactic inhibition of neutrophil elastase prevents the development of chronic neuropathic pain in osteoarthritic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Milind M Muley,Eugene Krustev,Allison R Reid,Jason J McDougall

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Native human alpha 1 Antitrypsin protein (Active)

Western blot - Native human alpha 1 Antitrypsin protein (Active) (AB91136)

SDS-PAGE - Native human alpha 1 Antitrypsin protein (Active) (AB91136)

주요 정보

Purity

발현 시스템

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

보관 버퍼

대체품

Proteins & Peptides

보완 제품

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Assay Kits

Reactivity 정보

제품 세부 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

Post-translational modifications

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise