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AB62415

Native Human Factor Va protein

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Native Human Factor Va protein is a Human Full Length protein with >95% purity and suitable for Functional studies and SDS-PAGE. The predicted molecular weight of ab62415 protein is 168 kDa (based on combined MW of subunits).

- Save time and ensure accurate results- use our Factor Va protein as a control
- Two subunits- Heavy chain: 94 kDa and light chain: 74 kDa
- Optimal protein bioactivity and stability

대체 명칭 보기

Coagulation factor V, Activated protein C cofactor, F5

1 Images
SDS-PAGE - Native Human Factor Va protein (AB62415)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Native Human Factor Va protein (AB62415)

SDS-PAGE analysis of ab62415 for 1ug protein.
Novex 4-12% Bis-Tris Gel
NR= non-reduced; R = reduced

주요 정보

Purity

>95%

Purity is determined by SDS-PAGE analysis

발현 시스템

Native

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

No

Accession

P12259

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

Constituents: 50% Glycerol (glycerin, glycerine), 0.0555% Calcium chloride

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Primary Antibodies

AB108614

Anti-Factor V antibody [EPR5191]

RabMAb|Recombinant

Western blot - Anti-Factor V antibody [EPR5191] (AB108614)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB283421

Recombinant Human IGHG1 Protein

SDS-PAGE - Recombinant Human IGHG1 Protein (AB283421)

  • 0
  • 0
Assay Kits

AB137976

Human Factor V ELISA Kit

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

The Native Human Factor Va protein ab62415 is prepared by activating purified factor V with thrombin and is subsequently purified by immunoaffinity chromatography. This process results in cofactor preparations which are free of both activation peptides and thrombin.

The Native Human Factor Va protein ab62415 is prepared from plasma shown to be non reactive for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II. Factor V is a very labile protein. Some degraded fragments are usually noticeable in preparations.

The bioactivity of Native Human Factor Va ab62415 is 4050 U/mg and was determined by Factor V clotting assay. One (1) unit is equivalent to the Factor V activity in one (1) milliliter of normal human plasma.

Ensure the validity of your result using our native human Factor Va protein ab62415 as a positive control in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here

Protein Determination: Extinction Coefficient (ε) 1% at 280 nm, 1cm pathway = 17.4

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서열 정보

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P12259","tags":[]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor Va also known as activated factor V plays a central role in blood coagulation. It forms a part of the prothrombinase complex which accelerates the conversion of prothrombin to thrombin an important step in the clotting process. Factor Va is a cofactor protein with a molecular mass of approximately 330 kDa. It is expressed primarily in platelets and plasma and its availability is increased during coagulation through activation by thrombin.
Biological function summary

Factor Va acts as an important component of the blood coagulation cascade. It forms a complex with factor Xa on phospholipid surfaces creating the tenase complex which is essential for the efficient conversion of prothrombin to thrombin. This complexation greatly increases the enzymatic efficiency of thrombin generation which is important for clot formation and stabilization. Factor Va's activity is regulated by activated protein C which inactivates factor Va to prevent excessive clotting.

Pathways

Factor Va significantly impacts the intrinsic and extrinsic pathways of blood coagulation. It directly interacts with factor Xa in the prothrombinase complex driving thrombin production. Thrombin in turn activates fibrinogen to fibrin promoting clot stabilization. The intrinsic and extrinsic pathways converge on factor Xa making its interaction with factor Va a shared critical point. This relationship emphasizes how factor Va integrates into broader coagulation processes.

Factor Va's role in the coagulation pathway makes it pivotal for conditions like thrombophilia and factor V Leiden. Anomalies in factor Va can lead to inappropriate clot formation contributing to thrombophilic disorders. The factor V Leiden mutation results from a change in the factor V gene rendering factor Va resistant to inactivation by activated protein C therefore increasing thrombotic risk. Understanding these interactions provides insights into pathologies involving coagulopathy.
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일반 정보

기능

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

서열 유사성

Belongs to the multicopper oxidase family.

Post-translational modifications

Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).. Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.. Activated protein C inactivates factor V and factor Va by proteolytic degradation.. Phosphorylated by FAM20C in the extracellular medium.

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제품 프로토콜

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타겟 정보

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
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Product promise

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