Recombinant Chinese hamster beta Actin protein (His tag)
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Recombinant Chinese hamster beta Actin protein (His tag) is a Chinese hamster Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.
대체 명칭 보기
Beta-actin, ACTB
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Chinese hamster beta Actin protein (His tag) (AB236171)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel using ab236171.
보완 제품
Primary Antibodies
AB8227
Anti-beta Actin antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 104
Primary Antibodies
AB16045
Anti-Cyclophilin B antibody - Loading Control
KO Validated|Lab Essentials
- 5
- 14
Reactivity 정보
서열 정보
특성 및 보관 정보
제형
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
일반 정보
기능
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (By similarity). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (By similarity). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (By similarity). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (By similarity). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
서열 유사성
Belongs to the actin family.
Post-translational modifications
Actin, cytoplasmic 1. N-terminal cleavage of acetylated methionine of immature cytoplasmic actin by ACTMAP.. ISGylated.. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.. Actin, cytoplasmic 1, N-terminally processed. N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.. Methylated at His-73 by SETD3 (By similarity). Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).
Subcellular localisation
Cytoskeleton
타겟 정보
Product promise
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